Molecular Detection of Antibiotic Resistance of Helicobacter pylori from Gastric Biopsies in Abidjan (Côte d'Ivoire)
Diplo Tchépe Flore Bernadette, C. Gbonon Mbengue Valérie, Guessennd Nathalie, Yapo Adou Francis, Kakou N'gazoa Solange, Ouattara Aboulaye, Coulibaly N'golo David, Djaman Allico Joseph, Dosso Mireille
Microbiology Research Journal International · pp. 1–7 · Published 9 May 2017
10.9734/MRJI/2017/32912Abstract
Aims: To determine the genes of resistance to amoxicillin, clarithromycin and metronidazole of Helicobacter pylori in gastric biopsies in Côte d'Ivoire. Place and Duration: The study was performed at the department of gastroenterology of Cocody Hospital and University Center, at the laboratory of Bacteriology-Virology and at the molecular biology platform of Pasteur Institute of Côte d'Ivoire from August 2015 to December 2016. Methodology: The rapid urease test was performed in endoscopy room and 98 positive biopsies were retained for the study. Gastric biopsies were collected and transported within a maximum of 4 hours. DNA extraction was followed by Polymerase Chain Reaction (PCR) amplification. Results: The rdxA / frxA, 23S rRNA and pbp1 genes conferring resistance to metronidazole, clarithromycin and amoxicillin respectively were identified in 12.2% (12/98), 26.5% (26/98) and 58.2% (57/98). Cross-resistance genotypes to these three antibiotics were detected in 8.2% (8/98) of the samples. Conclusion: These results show a high level of resistance of Helicobacter pylori to amoxicillin and presence of cross-resistance to the three commonly used antibiotics. These results support the need for an evaluation of Helicobacter pylori current therapeutic protocol in Côte d'Ivoire.
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Cited by 4
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