A Novel Ultra Performance Liquid Chromatographic Method for the Estimation of Lercanidipine in Bulk and Tablet Dosage Form
Satyabrata Sahu, U. Sunila Kumar Patra, Pratap Kumar Patra
Journal of Pharmaceutical Research International · pp. 768–776 · Published 16 Dec 2021
10.9734/jpri/2021/v33i59A34327Abstract
Aim: In this present study an accurate reverse phase ultra-performance liquid chromatography (RP-UPLC) method has been developed, validated and applied to stability indicating studies to determine Lercanidipine HCL in bulk and marketed dosage form. Methods: Optimized chromatographic conditions were achieved by using Waters Acquity BEH C18 (2.1 x 50mm, 1.7m) UPLC column. Empower 2 is a software, dihydrogen Orthophosphate Buffer : Methanol (40 : 60) as eluent at flow rate 0.3 ml/min. PDA detection was performed at 254nm. Results: The developed method was validated and stability study was conducted as per ICH guidelines. The retention time was found at 0.503 min. The method shows linearity over a range of 1 μg/ml to 60 μg /ml with the obtained correlation coefficient is 0.999. The LOD and LOQ values were found 0.025 and 0.05 μg /ml. The acidic and peroxide stressed study shows more degradation of 6.23% and 3.03%. Conclusion: The present developed method was found stability indicating, reliable, validated method was applied for the routine analysis of lercanidipine in bulk drug and the pharmaceutical formulations.
Cited by 1
Mahesh Ranga, Arun Kumar Modini, Anil Kumar Nallajarla · Journal of Molecular Structure · 2025
Related research
- A Review on Bioanalytical Method Development and Validation for Estimation of Drugs by Using Hyphenated Techniques and its Applications on Routine Analysis — shares topic coverage
- Sildenafil Determination by Using UPLC in Different Italian Viagra Tablets — shares topic coverage
- Application of Multi-criteria Decision Making Approach and Experimental Design and Development of Stability-indicating UPLC Method for Simultaneous Determination of Losartan Potassium and Hydrochlorothiazide in Bulk and Tablet Dosage Form — shares topic coverage
- Rapid, Simple and Validated Method for Estimation of Zolpidem by UPLC in Tablet Dosage Form — shares topic coverage
- Stability-Indicating RP-UPLC Method Development and Validation for the Process Related Impurities of Nebivolol and Structural Characterization of Its Forced Degradation Products by LC-MS/MS — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.