A Comparative Study on Anti-inflammatory Activity of Hydroethanolic of Leaf, Stem and Root Extracts of Tecoma stans

Abstract

Background: Tecoma stans is an important medicinal plant. The major bioactive compounds like alkaloids, phenols, terpenoids, glycosides, flavonoids and saponins had been isolated from this plant. The leaves bark and roots contain biologically active chemicals, and extracts from those tissues are in use as traditional folk medicines. Inflammation is a biological response of the immune system that can be triggered by a variety of factors, including pathogens, damaged cells and toxic compounds. The aim of the study was to analyze the anti-inflammatory activity of hydroethanolic leaf, stem and root extracts of Tecoma stans. Materials and methods: All chemicals and reagents were bought by sigma Chemicals Company. Anti inflammatory activity of the three different parts (leaf, stem and root) the plant were analysed by an in-vitro protein denaturation inhibition assays and inhibition calculation on protein inhibition percentage was done by the formula % inhibition=100−(A1 -A2)/A0 )*100). Statistical analysis was done by ANOVA and Duncan's multiple range test. Results: After comparing the anti-inflammatory activity of the leaf root stem of Tecoma stans, the results were plotted in graphs through statistical analysis using ANOVA and Duncan's multiple range test. The results showed that the stem of the plant has maximum anti-inflammatory activity compared to leaf and root. Conclusion: A comparative study of the extract or stem, leaf and root shows that the stem portion of the plant contains maximum anti-inflammatory activity.