Antimicrobial Resistance Profile and Molecular Characterization of Extended-spectrum Beta-lactamase Genes in Enterobacteria Isolated from Human, Animal and Environment
Gbonon M’bengue Valérie Carole, Guessennd Nathalie Kouadio, Ouattara Mohamed Baguy, Ouattara Gnoh Djénéba, Abraham Ayayi, Tiekoura Konan Bertin, Toty Abalé Anatole, Kouadio Kouamé Innocent, Dissinviel Stéphane Kpoda, Tahou Eric, Konate Ali, Konan Fernique, Kamenan Alphonse, Dosso Mireille, Ger Bmr
Journal of Advances in Microbiology · pp. 1–9 · Published 13 Apr 2018
10.9734/JAMB/2018/39955Abstract
Objective: The aim of this study is to determine the antibiotic resistance profile and characterize extended spectrum beta-lactamase gene of enterobacteria strains isolated from human biological products, fecal matter of animals and the environment. Materials and Methods: Enterobacteria producing ESBL strains were isolated from human products, fecal matter of healthy animals (cattle, sheep and pigs) intended for human consumption and environment (hospital effluents and municipal sewage) using homemade medium (Drigalski supplemented with 2 mg/L of ceftazidime). Resistance to beta-lactams has been evaluated by the diffusion method was carried out as recommended by NCCLS. Characterization of Beta-Lactamase resistance genes (blaCTXM, blaSHV, blaTEM, blaGES, blaPER and blaVEB) was performed by simplex and multiplex PCR. Results: The strains were resistant to antibiotics from beta-lactam family (penicillin with inhibitor, monobactam, cephalosporin) but no resistant was observed to carbapenem (imipénème, méropénème). All resistance genes were identified in environment strains. Conclusion: This study showed the presence of common beta-lactam resistance genes (blaTEM, blaSHV and blaCTX-M) to human, animal and environment. The risk of dissemination and circulation of ESBL enterobacteria between animals, humans and the environment exists in Ivory Coast because of the absence of a barrier between them.
Cited by 0
No indexed citations yet.
Related research
- Gastrointestinal Pathologies Recorded at the Felix Houphouet-boigny University Community Health Center from 2013 to 2017 (Abidjan - Ivory Coast) — shares topic coverage
- Morphological Diversity of Fungal Strains Responsible for Dessert Banana Rot, Musa Sapientum (Musaceae) Produced in the South Comoe Region on Ivory Coast — shares topic coverage
- Role of Healthy Human Gut Microbiota in the Emergence and Dissemination of Extended-Spectrum β-lactamase-Producing Enterobacteriaceae and Genes Associated with β-lactam Resistance in Community Settings in Abidjan, Côte d'Ivoire — shares topic coverage
- Assessment of Agricultural Practices and Socio-Economic Challenges in Urban Market Gardening: A Case Study of Yopougon, Ivory Coast — shares topic coverage
- Influence of Downward Tapping Delay on Agronomic Parameters of Upward Tapping Rubber Trees — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.