Antibiogram Analysis and Characterization of Bacterial Pathogens from Leucorrhea Patients

Abstract

In recent research work bacteria were isolated from samples of leucorrhea patients admitted in Lady Wellington hospital Lahore (gynae ward) and Basheer welfare hospital Shahdara Lahore. The sampling was done from pregnant and non-pregnant leucorrhea patients aged 18 to 30 years by using sterile culture sticks from vagina. The samples were spread on agar plates and incubated for overnight, bacterial strains were isolated by streak plate method. The strains were named L1, L2, L3 and L4. Identification was carried out by various morphological and biochemical tests. Molecular characterization was also done to characterize bacteria up to species level. L1 strain was identified as Streptococcus pyogens, L2 as Staphylococcus aureus, L3 as Neisseria gonorrheae and L4 as Escherchia coli. Antibiotic resistance was analyzed by disc plate method.  L1, L2 and L3 strains showed maximum sensitivity with Cefepime antibiotics having values 17.74 µg/ml, 13.63 µg/ml and 12 µg/ml respectively. L4 showed max sensitivity with Azithromycin and Cloxacillin antibiotics i.e., 6.25 µg/ml and 6.4 µg/ml respectively. Optimum pH was 6.5 for L1 and L2, while 7 for L3 and L4. Optimum temperature was 37 for all strains.