Evaluation of C. cassia Effectiveness in Behavioral Modulation of Haloperidol Induced Parkinson’s Disease (Mice Model)

Abstract

Aims: This study was undertaken to evaluate the effectiveness of behavioral modulation of C. cassia extract against Haloperidol induced Parkinson’s disease (PD) in albino mice. Study Design: The study used animal models to test the effect of Haloperidol in development of PD and effectiveness of C. cassia extract in behavioral modulation in mice before & after the treatment of PD. Place and Duration of Study: Pharmacology Lab, Department of Pharmacology, Faculty of Pharmacy, Hajvery University, Lahore-Pakistan. Methodology: The study was divided into three phases; During Phase I, all subjects were randomly divided into four groups comprising of 7 subjects each through flip of coin method and were trained for wire hanging test, grip strength test, Vertical rod test, and swim test for seven days. During Phase II, PD was induced by intraperitoneally administering 1 mg / kg / day of Haloperidol for 7 days. Animals of Group A (Normal) was served as control, Group B (Diseased, HP) received haloperidol (1 mg/kg / day), Group C (HP+CN-100), and Group D (HP+CN-200) were administered C. cassia extract, 100 mg and 200 mg per kg of body weight orally for 36 days respectively. During Phase III the above mentioned test were performed and effects of C. cassia extracts were recorded. The results of the present study revealed that dose of C. cassia extract 100 mg/kg is more safe and effective than 200 mg/kg. However, in swim test Group D (HP+CIN-200) is more statistically significant as compared to Group B (Diseased, HP) with P = .003. Conclusion: The current study concludes that oral administration of C. cassia extract modulates haloperidol induced behavioral changes in mice. The present study suggests that extract will be helpful as adjunct therapy along with standard therapy of Levodopa/Carbidopa.