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PCR

Papers (38)

First Report of Wound Infection with Bacillus pumilus in Iran

Hossein Azizi, Farnaz Kheirandish, Fatemeh Saleh & Mehdi Moazzami Gudarzi · Annual Research & Review in Biology · 2014

Aims: Bacillus pumilus is a spore-forming bacillus that causes infections which has been rarely reported among humans. This research was the first report of isolating B. pumilus from bed sore in a patient who was a rancher in Iran. Presentation of Case: In October 2012 a man with...

Open access Research Article 10.9734/ARRB/2014/10825

Isolation and Molecular Biological Characterization of Fowlpox Virus from Specimen of Cutaneous Nodular Lesions from Chickens in Several Regions of Tanzania

S. N. Masola, A. Mzula, H. N. Tuntufye, C. J. Kasanga & P. N. Wambura · Microbiology Research Journal International · 2014

Aim: To investigate molecular and evolutionary characteristics of genes of fowlpox virus (FWPV) isolates from chickens in Tanzania. Study Design:  Experimental. Place and Duration of Study: Faculty of Veterinary Medicine, Sokoine University of Agriculture, Morogoro, Tanzania; bet...

Open access Research Article 10.9734/BMRJ/2014/8420

Molecular Detection of Entamoeba histolytica in Different Water Sources of District Peshawar, Pakistan

Noor Ul Akbar, Sultan Ayaz, Shafiqur Rahman, Sanaullah Khan, Shahid Niaz Khan, Aga Asad Noor, Bibi Ibtesam Shagufta, Farzana Raza & Muhammad Waqar · Annual Research & Review in Biology · 2014

Many species of protozoa present in the alimentary canal of human beings causing diseases. Due to water-borne diseases about 3.5 million people including 3 million children die throughout the world as well as about 98% deaths occur through extensive water-borne outbreaks in the e...

Open access Research Article 10.9734/ARRB/2014/7935

Direct and Rapid Diagnosis of Extra-Pulmonary Tuberculosis in Archival Tissue by Nested and Touchdown PCR

L. F. Nimri, Z. Al-joulani & I. Matalka · Microbiology Research Journal International · 2014

Aims: To identify and differentiate mycobacterium spp. in archival formalin-fixed, paraffin-embedded tissue by PCR to supply additional differential diagnostic method for Mycobacterium infections, where tuberculosis had been tested for by histopathological methods but without cul...

Open access Research Article 10.9734/BMRJ/2014/8427

Determination of Vancomycin and Methicillin Resistance in Clinical Isolates of Staphylococcus aureus in Iranian Hospitals

Abbas Farhadian, Qorban Behzadian Nejad, Shahin Najar peerayeh, Mohammad Rahbar & Farzam Vaziri · Microbiology Research Journal International · 2014

Aims: The aim of this study was to determine the prevalence of methicillin resistant S. aureus (MRSA), vancomycin resistant or vancomycin intermediate resistant S. aurues (aureus) (VRSA/VISA) among clinical isolates. Study Design: S.aureus isolates used in this study were randoml...

Open access Research Article 10.9734/BMRJ/2014/4836

Lipolytic Activity and Molecular Identification of Pseudomonas aeruginosa and Lysinibacillus sphaericus Isolated from Domestic Oil Rich Wastewater

A. T. Odeyemi, B. I. Aderiye, E. I. Adeyeye, E. Donbraye & T. Faleye · Microbiology Research Journal International · 2014

Microbial lipases have been heightened in bioremediation and various industries. Place and Duration of Study: Department of Microbiology, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria, between September 2010 and August 2011. To identify the lipolytic enzyme producing mi...

Open access Research Article 10.9734/BMRJ/2014/6587

Molecular Detection of Giardia lamblia and Cryptosporidium parvum in Different Water Sources of District Bannu, Khyber Pakhtunkhwa, Province of Pakistan

Muhammad Shoaib Alam, Sana Ullah Khan, Sultan Ayaz, Noor Ul Akbar, Muhammad Asim Khan, Iftikhar Ahmad, Muhammad Idrees & Muhammad Waqar · Microbiology Research Journal International · 2013

Background: Cryptosporidium parvum and Giardia lamblia are intestinal parasites that predominantly causes "waterborne" infections that are transmitted through consumption of contaminated water. Both parasites typically cause an acute short-term infections with self-limiting diarr...

Open access Research Article 10.9734/BMRJ/2014/3947

Prevalence of Plasmid-Mediated ampC Genes in Clinical Isolates of Enterobacteriaceae from Cairo, Egypt

Nevine Fam, Doaa Gamal, Manal El Said, Inas El Defrawy, Ehab El Dadei, Soheir El Attar, Ashraf Sorur, Salwa Ahmed & John Klena · Microbiology Research Journal International · 2013

Aims: To determine the prevalence of acquired pAmpCs in clinically important and relevant enterobacterial species and to characterize the molecular types of pAmpC present in our geographic area. Methodology: Sixty Enterobacterial clinical isolates resistant to third generation ce...

Open access Research Article 10.9734/BMRJ/2013/4653