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PCR

Papers (38)

Antibiotic Susceptibility Test of Klebsiella pneumoniae and K. oxytoca Isolated from Different Clinical Samples and Perform Random Amplified Polymorphic DNA among K. pneumoniae

Surya Subedi, Jyoti Maharjan & Bidya Shrestha · Microbiology Research Journal International · 2016

Aims: To study the prevalence of MDR and ESBL producing Klebsiella pneumoniae and Klebsiella oxytocaand perform RAPD (Random amplified polymorphic DNA) by optimizing PCR among isolated ESBL producing Klebsiella pneumoniae. Place and Duration of Study: This study was done to asses...

Open access Research Article 10.9734/BMRJ/2016/23376

Isolation and Molecular Detection of Pathogenic Vibrio Species among Economic Fish from Red Sea in Egypt

Mohamed Wa el Abdel-Azeem, Ahmed Attaya, Manal I. El-Barbary & Serageldeen Sultan · Microbiology Research Journal International · 2016

A total of 105 samples were collected from Siganus rivulatus, Mulloidichthys vanicolensis, and Lethrinus lentjan, freshly captured from the Red Sea along Hurghada City coastline zone, Egypt. Clinical and post mortem findings revealed the presence of characteristic clinical signs...

Open access Research Article 10.9734/BMRJ/2016/24016

Sequence Variation in the rDNA Region of Root-Knot Nematodes (Meloidogyne spp) Infecting Indigenous Leafy Vegetables in Kisii and Transmara Sub-counties, Kenya

J. M. Mwangi, C. Gichuki, W. Wanjohi, S. Runo & P. K. Maina · Annual Research & Review in Biology · 2016

Aim: The aim of the study was to evaluate the utility of DNA sequencing in differentiating Root-Knot Nematodes (RKN) (Meloidogyne spp) infecting Indigenous Leafy Vegetables (ILVs) in Kisii and Transmara sub-counties in Kenya. Study Design: Cross-sectional study. Place and Distrib...

Open access Research Article 10.9734/ARRB/2016/17985

Molecular Biology Methods to Assess Microbiological Hazard Concerning Objects of Cultural Heritage

Tomasz Lech · Microbiology Research Journal International · 2015

Microorganisms present in the environment pose a considerable hazard to historical objects and works of art. They are capable of settling on objects, and due to their ability to produce various enzymes, they can actively destroy these special objects, the poor preservation of whi...

Open access Research Article 10.9734/BMRJ/2015/19535

Prevalence of Enteropathogenic Escherichia coli (EPEC) in Adult Diarrhea Cases and their Antibiotic Susceptibility Pattern

S. Mariyam Sunaifa, Somdatta Roy & B. Dhanashree · Microbiology Research Journal International · 2015

Introduction: Diarrheagenic Escherichia coli (DEC), an important etiologic agent of diarrhea is a major public health problem in developing countries. Relatively few studies have reported the role of enteropathogenic E. coli (EPEC) as etiological agent of adult diarrhea. Objectiv...

Open access Research Article 10.9734/BMRJ/2015/17468

Genetic Diversity Study of Locally Isolated Bacillus thuringiensis Strains from Kuwait Using Random Amplified Polymorphic DNA Analysis

Jafar A. Qasem, Ebtesam M. Al-Ali, Salwa A. Al-Mouqati & Yousif A. Al-Shayji · Microbiology Research Journal International · 2015

Introduction: Bacillus thuringiensis (Bt) is significant bacteria in the field of insect biological control due to their insecticidal properties and its importance in agriculture. Aim: The objective of this study was to analyze genetic variation of 15 native isolates of Bacillus...

Open access Research Article 10.9734/BMRJ/2015/17009

Molecular Epidemiology of Shiga Toxin-Producing Escherichia coli Isolated from School Children in Ondo State, Nigeria

A. K. Onifade & M. A. Oladoja · Microbiology Research Journal International · 2015

Molecular techniques were used for studying the epidemiology of Escherichia coli from five major towns in Ondo state, namely; Akure and Ondo, Owo, Okiutipupa and Ikare-Akoko. In total, 807 urine and faecal samples of apparently healthy primary school pupils were gathered between...

Open access Research Article 10.9734/BMRJ/2015/14535

Differentiation in a Single-Tube PCR between Leishmania mexicana and Leishmania braziliensis in Clinical Samples

Victor Monteon, Enrique Quen-Ramirez, Román Diaz-Arce, María Vargas, Ruth López, Lidia Baylon & José L. Rosales · Microbiology Research Journal International · 2015

Aim: To assess the usefulness of a single-tube PCR end point for differentiating between Leishmania and Viannia subgenera in clinical samples of cutaneous leishmaniasis. Methodology: Impression smears of 65 patients from Campeche State, Mexico with leishmaniasis-like lesions were...

Open access Research Article 10.9734/BMRJ/2015/12418

Occurrence of Giardia in Different Water Sources in District Bannu

Shahid Niaz Khan, Sultan Ayaz, Sanaullah Khan, Ibrar Ullah, Shahid Ullah, Noor Ul Akbar, Muhammad Asim Khan, Sobia Attaullh, Jabbar Khan & Ijaz Ali · Microbiology Research Journal International · 2014

Background: After air, water is one of the greatest significant essentials for life, which is considered as one of the nutrients. Giardia lamblia (G. lamblia) is one of the most common waterborne protozoan parasites, causing diarrheal disease in human beings and animal diseases t...

Open access Research Article 10.9734/BMRJ/2015/11527

Influenza and Respiratory Syncytial Virus in Hospitalized Patients with Acute Exacerbation of Severe COPD in Izmir, Turkey

Gulrü Erbay, Ceyda Anar, Gunes Senol, Can Bicmen, Yusuf Pinar & Melih Buyuksirin · Microbiology Research Journal International · 2014

Aims: The aim of this study is documenting the presence of respiratory syncytial virus (RSV) and influenza virus infections in hospitalized patients with AE-COPD in autumn and winter months. Study Design: The study was designed as a prospective cohort study. Place and Duration of...

Open access Research Article 10.9734/BMRJ/2015/13251

Phenotypic and Molecular Characterization of MBL Genes among Uropathogens Isolated in Mumbai City

Tariq Mobashshera & K. Aruna · Microbiology Research Journal International · 2014

Aims: Growing incidences of the pathogens producing Metallo-β-lactamases (MBLs) has been observed in many countries including India. They are carbapenemases that are capable of hydrolysing carbapenems that are often considered as a last resort antibiotic, for infections caused by...

Open access Research Article 10.9734/BMRJ/2015/13762

Biochemical and Molecular Characterization of Food Contaminating Bacteria Isolates from Food Stall Vegetables

Ali Ramadan Ali ALatawi, Sutarno ., Ari Susilowati & Hailu Weldekiros Hailu · Microbiology Research Journal International · 2014

Today the issue of food safety is global problem that gets main concern in setting public health policy. The eruption of diseases caused by food contamination occurs in places where sanitation and hygiene conditions are generally poor. Reliable identification of bacteria remains...

Open access Research Article 10.9734/BMRJ/2015/13792

Molecular Characteristics of Community-Associated Methicillin-resistant Staphylococcus aureus (CA-MRSA) Isolates from Clinical Specimens in Iraq

Alaa H. Al-Charrakh, Huda H. Al-Hassnawi & Jawad K. Al-Khafaji · Microbiology Research Journal International · 2014

Background: Methicillin-resistant Staphylococcus aureus (MRSA) infections have been recognized for decades as hospital acquired MRSA (HA-MRSA). Nowadays, MRSA is also recognized as a worldwide emerging community-associated pathogen. Community associated-MRSA (CA-MRSA) has been sh...

Open access Research Article 10.9734/BMRJ/2015/9607

Detection of Pathogenic Legionella pneumophila in Tap Water and Water of Newborn Incubators in Hospitals of Guilan Province by PCR of mip Gene

Masoumeh Ahmadi Jalali Moghadam, Hamidreza Honarmand, Sajad Asfaram Meshginshahr, Bahram Soltani Tehrani & Majid Nojavan · Microbiology Research Journal International · 2014

Aims: This study is designed to determine the frequency of Legionella pneumophila in cold and warm water as well as water containers of newborn incubators in Guilan province hospitals, Iran, using amplification of the macrophage infectivity protein gene (mip gene) by PCR. Study D...

Open access Research Article 10.9734/BMRJ/2014/9830

Molecular Detection and Antimicrobial Activity of Enterolysin a Endopeptidase among Enterococcus faecalis Strains of Different Origins

Mitra Salehi & Zeinab Hatami · Annual Research & Review in Biology · 2014

Aims: The target of present study is to determine the frequency of occurrence of enterolysin A gene in Enterococcus faecalis strains from different sources and surveys about antibacterial effect of enl A gene positive strains. Study Design: Enterolysin A is a class IV bacteriocin...

Open access Research Article 10.9734/ARRB/2014/7216