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All published articles 904

Efficient Micropropagation of Spilanthes acmella (L.) Murr.: A Threatened Medicinal Herb

Shiwali Sharma & Anwar Shahzad · Biotechnology Journal International · 2013

The present study describes an efficient and reproducible protocol for micropropagation of S. acmella. Shoot tips taken from 3 week-old aseptic seedlings were cultured on Murashige and Skoog (MS) semi-solid medium supplemented with different concentrations of TDZ. Among various c...

Open access Research Article 10.9734/BBJ/2013/3556

Characteristics of Penicillin G Acylase Immobilized onto Iron Oxide Nanoparticles

Momen Seyed Bahman, Atyabi Seyed Mohammad, Azim Akbarzadeh, Salimi Mona, Sedigeh Hatami Gigloo, Farhangi Ali & Norouzian Dariush · Biotechnology Journal International · 2013

Penicillin G acylase was immobilized onto iron oxide nanoparticles coated with polyethyleneimine and then cross linked with glutaraldehyde solution. The FTIR spectrum of immobilized enzyme showed peak at 1648cm-1 which can be attributed to the C=N bonds of Schiff’s base linkage f...

Open access Research Article 10.9734/BBJ/2013/2987

In vitro and In vivo Effects of the Leaf Extracts of Cassia tora and Cassia sophera in Reducing the Cytotoxicity and Angiogenesis

Sumangala Rao & C. Suresh · Biotechnology Journal International · 2013

Aim: Consumption of C. tora and C. sophera of family caesalpinaceae as leafy vegetables by local folklore has initiated this study to investigate the cytotoxic effect and antiangiogenic effect of their leaf extracts on human cancer cell lines and chick embryo respectively. Study...

Open access Research Article 10.9734/BBJ/2013/4244

SSR- Based Genetic Diversity Assessment in Tetraploid and Hexaploid Wheat Populations

Hanaa Mahdy Abouzied, Samah M. M. Eldemery & Kamal Fouad Abdellatif · Biotechnology Journal International · 2013

Molecular analysis for a set of hexaploid (Triticum aestvium) and tetraploid (Triticum durum) wheat cultivars was investigated by applying 11 SSR primers set. The plant materials consisted of 45 genotypes 15 of which were Triticum aestivum and 30 of T. durum obtained from four di...

Open access Research Article 10.9734/BBJ/2013/4340

Growth Inhibition of Some Phytopathogenic Bacteria by Cell-Free Extracts from Enterococcus sp

Luis C. Mata, Catalina Chavez, Raúl Rodríguez-Herrera, Daniel Hernández-Castillo & Cristóbal N. Aguilar · Biotechnology Journal International · 2013

Aims:  To inhibit of bacterial growth of three important phyto-pathogenic bacteria: Erwinia carotovora, Clavibacter michiganensis sp. michiganensis and Xanthomonas axonopodis by cell-free extracts from submerged cultures of two strains of Enterococcus sp. was tested. Study Design...

Open access Research Article 10.9734/BBJ/2013/3024

Light Microscopic and Immunohistochemical Study Revealed Sex-Dependent Kidney Morphology in DBA/2crSlc Mice

M. A. Awal, A. Yabuki, O. Ichii, M. Matsumoto, S. Suzuki & J. Alam · Biotechnology Journal International · 2013

Aim: Sex-dependent differences in kidney histology have been observed in different species of the laboratory animals. The present study was conducted to evaluate the sex and strain-dependent changes in DBA/2CrSlc mouse kidney morphology by using light microscopy and immunohistoch...

Open access Research Article 10.9734/BBJ/2013/3100

Antibacterial and Antiviral Activities of Essential Oils of Northern Moroccan Plants

Naima El Moussaoui, Gloria Sanchez, El Ouardy Khay, Mohammed Idaomar, Ahmed Ibn Mansour, Jamal Abrini & Rosa Aznar · Biotechnology Journal International · 2013

Aims: This study was designed to evaluate the antimicrobial activity of five essential oils (EOs) extracted from the aerial parts (leaves and flowering tops) of three species growing in the north of Morocco: Origanum elongatum, Thymus capitatus and Mentha suaveolens. Study Design...

Open access Research Article 10.9734/BBJ/2013/3596

Bacteriological Study of Urinary Tract Infections with Antibiotics Susceptibility to Bacterial Isolates among Honeymoon Women in Al Qassim Hospital, Babylon Province, Iraq

Nada Khazal Kadhim Hindi, Shaimaa O. Hasson & Shatha Khazal Kadhim Hindi · Biotechnology Journal International · 2013

Urinary Tract Infections (UTIs) is an infection caused by the presence and growth of microorganism anywhere in the urinary tract. This study aims at determining the most common microorganism that causes UTI in honeymoon women, detection the most effective antimicrobial agents tha...

Open access Research Article 10.9734/BBJ/2013/3573

Screening of Locally Isolated Aspergillus species for Their Cellulolytic Potential and Their Optimization on Vigna mungo in Solid State Fermentation

Umbrin Ilyas, Faryal Gohar, Sumaria Saeed, Zanib Bukhari & Hira Ilyas · Biotechnology Journal International · 2013

Aims: To evaluate cellulolytic potential of locally isolated Aspergillus species and emphasis the importance of screening on qualitative bases. Also, to enhance cellulase production by optimizing the process parameters of the solid state fermentation of Vigna mungo with the isola...

Open access Research Article 10.9734/BBJ/2013/2849

Thyroid Hormone Profile in Rwandan Students

Jean Bosco Gahutu · Biotechnology Journal International · 2013

Aim: To illustrate the thyroid hormone profile in young adult students at moderate altitude in Rwanda. Study Design: A population-based cross-sectional study. Place and Duration of Study: The study was conducted among healthy university students living at Butare, Rwanda (altitude...

Open access Research Article 10.9734/BBJ/2013/3231

Effect of Gas Flaring on the Phytochemical and Nutritional Composition of Treculia africana and Vigna subterranean

C. O. Ujowundu, L. A. Nwaogu, F. N. Ujowundu & D. C. Belonwu · Biotechnology Journal International · 2013

Aims: The effect of petroleum exploration and exploitation activities on the phytochemical and nutritional composition of African breadfruit (Treculia africana) and Bambara groundnuts (Vigna subterranean) harvested from polluted and unpolluted environment were studied. Study Desi...

Open access Research Article 10.9734/BBJ/2013/3033

Adsorption of Copper (II) Ions from Aqueous Solution onto Synthetic Goethite and Two Naturally Available Red Soils from Yaoundé –Cameroon

Tagne Guy Merlain, NDI Julius Nsami & Ketcha Joseph Mbadcam · Biotechnology Journal International · 2013

The present investigation deals with the utilization of synthetic goethite and two red soils obtained from AHALA-NSAM and NKOLOFANE as adsorbent for the removal of copper from waste water. The synthetic goethite was characterized by IR, XRD and BET where the red soils were charac...

Open access Research Article 10.9734/BBJ/2013/3117

Application of Box-Behnken Design for the Optimization of Citric Acid Production from Corn Starch Using Aspergillus niger

N. A. Amenaghawon, K. I. Nwaru, F. A. Aisien, S. E. Ogbeide & C. O. Okieimen · Biotechnology Journal International · 2013

Citric acid production from hydrolysed corn starch was optimized in this study. Response surface methodology (RSM) was employed for the analysis of the simultaneous effect of substrate concentration, broth pH and fermentation temperature on the concentration of citric acid produc...

Open access Research Article 10.9734/BBJ/2013/3379