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All published articles 904

Morphological Anomalies in Somatic Embryo Structure in Catharanthus roseus: Improving Embryo Germination by Amending Plant Growth Regulators, Activated Charcoal and Sucrose Level

Dipti ., Samar Fatima & Abdul Mujib · Biotechnology Journal International · 2013

The present study describes the incidence of somatic embryo (SE) irregularities in Catharanthus roseus (L.) G. Don. In this ornamental and anti-cancerous medicinal plant, SEs were developed at a very high rate from hypocotyl callus on MS medium, amended with  1.0 mg l-1 naphthale...

Open access Research Article 10.9734/BBJ/2014/4809

Effects of Utilization of Crushed, Boiled and Fermented Roselle Seeds (Hibiscus sabdariffa) on the Performance of Broiler Chickens

Maikano Mohammed Ari, Danlami Moses Ogah, Idris Danladi Hassan, Ibrahim Suleiman Musa-Azara, Nuhu Dalami Yusuf & Samuel Emmanuel Alu · Biotechnology Journal International · 2013

Aim of Study: The study examines the effects of utilization of crushed (CRRS), boiled (HTRS) and fermented (FRS) Roselle seeds (Hibiscus sabdariffa) on the performance of broiler chickens Study Design: A total of 135 Anak day-old broiler chicks were randomly assigned to three (3)...

Open access Research Article 10.9734/BBJ/2014/6061

Histopathological Effects of Glyphosate and Its Toxicity to the Earthworm Nsukkadrilus mbae

Oluah Ndubuisi Stanley & Ochulor Amarachi Joy · Biotechnology Journal International · 2013

This study was carried out to investigate the toxicity of Roundup, aglyphosate-containing herbicide to the earthworm Nsukkadrilus mbae and the associated histological changes. One hundred and sixty five earthworms were randomly divided into five groups and exposed to different co...

Open access Research Article 10.9734/BBJ/2014/6727

Haploid Plant Regeneration from Unpollinated Ovules of Cucumis melo L. Var. Conomon cv. Mudicode

Sunita Premnath Koli & Hosakatte Niranjana Murthy · Biotechnology Journal International · 2013

Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultured in vitro on MS medium supplemented with IAA, IBA, NAA, 2,...

Open access Research Article 10.9734/BBJ/2013/5417

L-Phenylalanine and L-Tyrosine Catabolism by Thermophilic Geobacillus stearothermophilus

M. Afzal, S. Al-Awadhi & S. Oommen · Biotechnology Journal International · 2013

Aims: This study investigated the potential of soil thermophile Geobacillus stearothermophilus for the biotransformation of phenylalanine and tyrosine. Study Design: G. Stearothermopilus grows well at 65ºC and has a good potential for transformation and biodegradation of many com...

Open access Research Article 10.9734/BBJ/2013/4516

Genetic Characterization of Insulin Growth Factor-1 and Its Receptor Genes in Egyptian Buffalo (Bubalus bubalis L.)

Othman E. Othman, Mohamed F. Abdel-Samad, Nadia A. Abo El Maaty & Karima M. Sewify · Biotechnology Journal International · 2013

Aim: The somatotropic axis (SA) comprises genes associated with economically important quantitative traits in livestock like mammary and muscle growth as well as carcass traits. Insulin growth factor-1 (IGF-1) and its receptor (IGF-1R) are two important genes belonging to the SA....

Open access Research Article 10.9734/BBJ/2013/4869

Characterization of Dairy Effluents by Physicochemical Parameters

Vishakha Sukhadev Shivsharan, Minal Wani & M. B. Khetmalas · Biotechnology Journal International · 2013

Aims: The dairy industry faces growing scrutiny of its environmental stewardship.  The potential impact of an individual operation on the environment varies with animal concentration, weather, terrain, soils, and numerous other conditions. It is hoped that management practices fo...

Open access Research Article 10.9734/BBJ/2013/3640

Improved Sample Preparation for PCR-Based Assays in the Detection of Xanthomonads Causing Bacterial Leaf Spot of Tomato

E. R. Mbega, J. Adriko, C. N. Mortensen, E. G. Wulff, O. S. Lund & R. B. Mabagala · Biotechnology Journal International · 2013

Aims: To develop a sampling procedure for PCR-based screening of bacterial leaf spot (BLS)-causing xanthomonads without DNA extraction from infected tomato plants. Place and Duration of Study: University of Copenhagen, Denmark and Sokoine University of Agriculture, Morogoro, Tanz...

Open access Research Article 10.9734/BBJ/2013/3810

Dynamic Principle of Center of Mass in Human Walking

Yifang Fan, Yubo Fan, Zhiyu Li, Mushtaq Loan & Changsheng Lv · Biotechnology Journal International · 2013

Assuming the ground reaction force of both feet to be the same in the same phase of a stride cycle, we establish the relationships between the time of initial foot contact and the ground reaction force, acceleration, velocity, displacement and average kinetic energy of center of...

Open access Research Article 10.9734/BBJ/2013/4724

Kinetic Study of Neem Biodiesel Production

M. Elkadi, A. E. Pillay, J. Manuel, S. Stephen & M. Z. Khan · Biotechnology Journal International · 2013

Aims: The kinetic features of neem biodiesel production were studied to establish the conversion parameters that govern optimal product yield in minimum reaction span. The mechanistic features of the dual acid/base catalytic conversion were investigated to gain an insight into th...

Open access Research Article 10.9734/BBJ/2013/4434

Optimization of Cellulase (E.C. 3.2.1: 4) Production Using Penicillium citrinum MTCC 9620 in Solid State Fermentation

G. Ghoshal, U. C. Banerjee & U. S. Shivhare · Biotechnology Journal International · 2013

Aim: The objective of the present work was to optimize the environmental parameters for Cellulase (1,4 β-endoglucanase, E.C.3.2.1:4) production using Penicillium citrinum MTCC 9620 in Solid State Fermentation. Study Design: One unit of Cellulase (1,4 β-endoglucanase, E.C.3.2.1:4)...

Open access Research Article 10.9734/BBJ/2013/4135

Constraining the Pluripotent Fate of Human Embryonic Stem Cells for Tissue Engineering and Cell Therapy – The Turning Point of Cell-Based Regenerative Medicine

Xuejun H. Parsons · Biotechnology Journal International · 2013

To date, the lack of a clinically-suitable source of engraftable human stem/progenitor cells with adequate neurogenic potential has been the major setback in developing safe and effective cell-based therapies for regenerating the damaged or lost CNS structure and circuitry in a w...

Open access Research Article 10.9734/BBJ/2013/4309

Establishment of Agrobacterium-Mediated Transformation System in Sweet Potato (Ipomoea batatas) by Culture of Leaf Segments for Functional Analysis of ASG-1, an Apomixis-Specific Gene

Lanzhuang Chen, Chengti Xu, Zhaosheng Du, Takuro Hamaguchi, Toru Sugita, Hiroaki Ichikawa & Liming Guan · Biotechnology Journal International · 2013

Aims: In order to do the functional analysis of apomixis-specific gene (ASG-1), which was isolated from apomictic guineagrass, the sweet potato was used to establish an Agrobacterium-mediated transformation system. Study Design: At first, plant regeneration was achieved from the...

Open access Research Article 10.9734/BBJ/2013/4582

Treatment of Wastewaters Generated by Surfactant-Enhanced Washing of Soils in an Aerobic Biofilter Inoculated with a Consortium of Hydrocarbon Degraders

M. Zacarías-Salinas, E. I. Garcia-Peña & L. G. Torres · Biotechnology Journal International · 2013

Aims: The aims of this work were 1) To evaluate the performance of a submerged biofiltration system for the treatment of a surfactant-enriched wastewater that had been generated by a soil washing process. 2) To evaluate the effect of the flux and organic load over the performance...

Open access Research Article 10.9734/BBJ/2013/4010