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All published articles 904

Structural Analysis and Docking of Stilbene Synthase Protein from Chinese Grape Vine Vitis pseudoreticulata

K. Divya, G. Venkata Ramana & K. V. Chaitanya · Biotechnology Journal International · 2014

Aims: The present work aims to perform the molecular modeling of stilbene synthase protein from Chinese grape vine Vitis pseudoreticulata. Place and Duration of Study: The study has been performed in the Department of Biotechnology, GITAM Institute of Technology, GITAM University...

Open access Research Article 10.9734/BBJ/2014/3835

Exploration of Actinobacteria from Mangrove Ecosystems of Nizampatnam and Coringa for Antimicrobial Compounds and Industrial Enzymes

Usha Kiranmayi Mangamuri, Muvva Vijayalakshmi & Sudhakar Poda · Biotechnology Journal International · 2014

Aim: A study was made to examine the kinship between the seasonal distribution of actinobacteria and the physico-chemical properties of the mangrove sediments of Nizampatnam and Coringa located along the South East coast of Andhra Pradesh, India. Place and Duration of Study: Depa...

Open access Research Article 10.9734/BBJ/2014/6424

In vitro Propagation of Caralluma diffusa (Wight) N.E.Br

K. Kalimuthu, K. Kalaiyarasi, R. Prabakaran & T. Sasikala · Biotechnology Journal International · 2013

An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Caralluma diffusa, via enhanced axillary bud proliferation from nodal explants collected from young shoots of six-months-old plant. The physiological effects of growth regul...

Open access Research Article 10.9734/BBJ/2014/7488

Effect of Desiccation and Chilling Treatment on Somatic Embryo Development and Germination in Rough Lemon (Citrus jambhiri Lush)

Balwinder Singh · Biotechnology Journal International · 2013

The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon (Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract fo...

Open access Research Article 10.9734/BBJ/2014/6241

Agrobacterium-Mediated Transfоrmation of Maize with Antisense Suppression of the Proline Dehydrogenase Gene by an In Planta Method

Y. M. Moiseeva, V. A. Velikov, I. V. Volokhina, Yu. S. Gusev, O. S. Yakovleva & M. I. Chumakov · Biotechnology Journal International · 2013

Aims: The aim of this study was to estimate the efficiency of T-DNA transfer during Agrobacterium-mediated transformation of maize (Zea mays L.) at different temperatures. In addition, the way of T-DNA transfer was studied after application of an Agrobacterium suspension at maize...

Open access Research Article 10.9734/BBJ/2014/6504

Heavy Metals Concentration in Fish Mugil cephalus from Machilipatnam Coast and Possible Health Risks to Fish Consumers

P. V. Krishna, K. Madhusudhana Rao, V. Swaruparani & D. Srinivas Rao · Biotechnology Journal International · 2013

Heavy metals are dangerous to aquatic organisms and it can be bioaccumulated in the food chain leading to diseases in humans. Cumulative effects of metals or chronic poisoning may occur as a result of long term expore even to low concentrations. The accumulation of heavy metals c...

Open access Research Article 10.9734/BBJ/2014/4317

3D Structure Modeling of Human Telomere Repeat Binding Factor 2 and DNA-Protein Docking Studies

Koel Mukherjee, Dev Mani Pandey & Ambarish Saran Vidyarthi · Biotechnology Journal International · 2013

Aims: Human telomere repeat binding factor (hTRF2) is a double stranded telomere binding protein that plays key role in protecting the chromosome ends and a necessary building block of telomere structure maintenance. The aim of the present study was to focus on the modeling of 3D...

Open access Research Article 10.9734/BBJ/2014/6381

In vitro Propagation and GC-MS Studies of Ocimum basilicum Linn. var. pilosum (Willd.) Benth

Gaddaguti Venu Gopal, Srideepthi Repalle, Venkateswara Rao Talluri, Srinivasa Reddy Ronda & Prasada Rao Allu · Biotechnology Journal International · 2013

An in vitro propagation method is outlined for Ocimum basilicum Linn. var. pilosum (Willd.) Benth., a wild aromatic plant belongs to Lamiaceae family. Shoot buds were used as source of explants on MS media supplemented with different concentrations of growth regulators for callus...

Open access Research Article 10.9734/BBJ/2014/6225

Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix

Jagdish Singh & Manpreet Kaur · Biotechnology Journal International · 2013

Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed batch study. Methodology: Effect of pH, matrix size, enzyme concentration, contact time and temper...

Open access Research Article 10.9734/BBJ/2014/6772

Establishment of an Efficient and Practical Virus-free Seedling Supply System by Means of Culture of Shoot Apexes, RT-PCR and Clonal Propagation in Sweet Potato (Ipomoea batatas)

Lanzhuang Chen, Chenti Xu, Zhaosheng Du, Takuro Hamaguchi, Toru Sugita, Ryutarou Nagata & Liming Guan · Biotechnology Journal International · 2013

Aims: Sweet potato (Ipomoea batatas) cv. “Miyazakibeni” was used as material for shoot apex culture, reverse transcription-polymerase chain reaction (RT-PCR) and clonal propagation to establish an efficient and practical virus-free seedling supply system in production of vegetati...

Open access Research Article 10.9734/BBJ/2014/6235

Different Methods for DNA Extraction from Yeast-Candida famata Isolated from Toddy

T. Santra, S. K. Ghosh & A. Chakravarty · Biotechnology Journal International · 2013

Aims: Isolation and biochemical characterization of yeasts from toddy and standardization of best method for DNA extraction from yeast. Study Design: Biochemical characterization of yeast and genomic DNA extraction by manual and kits methods. Place and Duration of Study: Departme...

Open access Research Article 10.9734/BBJ/2014/4884

Screening of Filamentous Fungi for Xylanases and Cellulases Not Inhibited by Xylose and Glucose

L. F. C. Ribeiro, L. F. Ribeiro, J. A. Jorge & M. L. T. M. Polizeli · Biotechnology Journal International · 2013

Aims: Screening different filamentous fungi for thermostable xylanases and cellulases that would not be inhibited by xylose and glucose, respectively. Methodology: Samples of fungi collected in the Atlantic forest region, Minas Gerais, Brazil, and some fungi from our Culture Coll...

Open access Research Article 10.9734/BBJ/2014/6066

Identification of Genes Putatively Involved in the Biosynthesis of Antitubercular Peptide in Streptomyces ribosidificus NRRL B-11466

Khaled M. Aboshanab, Nisreen M. Okba, Tarek S. El-banna & Ahmed A. Abd El-Aziz · Biotechnology Journal International · 2013

Aims: To determine the potential antitubercular activity of Streptomyces ribosidificus NRRL B-11466 both on genotypic and phenotypic levels.  Methodology: Standard methods and software programs were used for nucleotide/protein sequence analysis and phenotypic detection of antitub...

Open access Research Article 10.9734/BBJ/2014/5210

Activity of β-Amylase in Some Fungi Strains Isolated from Forest Soil in South-Western Nigeria

O. A. Oseni & M. M. Ekperigin · Biotechnology Journal International · 2013

Aims: The aim of this study was to isolate fungi species from Omo natural forest soil in Ogun State of Nigeria and study the amylases from the fungi species which are digestive enzymes that hydrolyze glycosidic bonds in starch to glucose, maltose, maltotriose and dextrin; and in...

Open access Research Article 10.9734/BBJ/2014/4686