α-Glucan phosphorylase is an important enzyme of carbohydrate metabolism. In spinach leaves, it has been reported in two multiple forms viz. Pho 2 (cytosolic) and Pho 1 (plastidial). Here, we extracted and purified Pho 2 form of α-glucan phosphorylase from spinach using salting o...
Open access
Research Article10.9734/BBJ/2015/15106
Aim: To identify isolates for industrial production of pectinase. Methodology: The isolates were screened for pectinolytic activity using pectin as substrate. Enzyme activity was expressed as mg of glucose equivalent released per ml of crude enzyme solution per second. The kineti...
Open access
Research Article10.9734/BBJ/2015/11449
Microsatellite DNA Marker Analysis Revealed Low Levels of Genetic Variability in the Wild and Captive Populations of Cirrhinus cirrhosus (Hamilton) (Cyprinidae: Cypriniformes) Aims: To reveal the genetic variability of wild and captive populations of the Indian major carp, mrigal...
Open access
Research Article10.9734/BBJ/2015/14895
Identification of Suitable Condition for Mannanase Production by Bacillus sp. GA2(1) Aims: The effects of 1% (w/v) supplementation of additional 5 agricultural wastes, corn cob, bagasses, coffee residues, soybean meal, and copra meal for mannanase production by Bacillus sp.GA2(1)...
Open access
Research Article10.9734/BBJ/2015/15121
Evaluation of Hypoglycemic Efficacy of Methanolic Extracts of Moringa Oleifera and Phyllanthus amarus in Diabetic Rats Aims: This study was undertaken to determine the hypoglycemic efficacy of methanolic extracts of Moringa oleifera and Phyllanthus amarus in alloxan-induced diabe...
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Research Article10.9734/BBJ/2015/13164
Hayat M. Farah, Tigani H. Elamin, Hassan E. Khalid, Abdel Rahim M. El. Hussein, Zakia A. Mohammed & Husna M. El Basheir·Biotechnology Journal International·2014
In vitro Antitheilerial Activity of Paluther (Artemether 80) Against Theileria lestoquardi Aim: The aim of this study was to screen Artemether 80 for activity against Theileria lestoquardi (Apicomplexa: Theileridae) using buparvaquone as a standard drug. Study Design: In vitro st...
Open access
Research Article10.9734/BBJ/2015/10605
With the growing population and subsequent increasing demand for energy in Nigeria, coupled with the under-developed electrical energy platforms and the vast problems of the convectional energy sources such as continuous gas flaring, energy crisis is still lingering and enormous....
Open access
Research Article10.9734/BBJ/2015/14333
Genotyping and Nucleotide Sequences of Growth Hormone Releasing Hormone and Its Receptor Genes in Egyptian Buffalo Aim: The hypothalamic hormone, growth hormone-releasing hormone, is the principal stimulator of pituitary growth hormone (GH) synthesis and secretion. GHRH and its r...
Open access
Research Article10.9734/BBJ/2015/11619
Effect of kLa and Fed-batch Strategies for Enhanced Production of Xylitol by Debaryomyces nepalensis NCYC 3413 Aims: To evaluate the effect of volumetric oxygen transfer coefficient on the production of xylitol by Debaryomyces nepalensis and to enhance the yield and productivity...
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Research Article10.9734/BBJ/2015/14273
Mina Khanbagi Dogahe, Kianoush Khosravi-Darani, Azadeh Tofighi, Mehrnaz Dadgar & Amir Mohammad Mortazavian·Biotechnology Journal International·2014
Effect of Process Variables on Survival of Bacteria in Probiotics Enriched Pomegranate Juice Aims: The consumption of foods and beverages containing probiotic microorganisms is a growing, global consumer trend. In this research, production of probiotic pomegranate juice containin...
Open access
Research Article10.9734/BBJ/2015/12114
Hailu Weldekiros Hailu, Viktor Abdallah, Ari Susilowati, Saleh Muhammed Raqib & Ali Ramadan Ali ALatawi·Biotechnology Journal International·2014
Biochemical Characterization, 16S rRNA Sequence Analysis and Multiple Sequence Alignment of Bacteria Isolated from Fermented and Unfermented Coconut Coconut (Cocos nucifera L.) is a tropical and subtropical plant which has great versatility due to many benefits of its different p...
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Research Article10.9734/BBJ/2015/14523
Effect of Arsenic (As) on the Spermatogenesis of Black Bengal Goat (Capra hircus) Reared at the Arsenic Prone Area of Mymensingh District in Bangladesh Aim: Arsenic (As) alters the spermatogenic process as well as testicular histology has been reported in different species of dom...
Open access
Research Article10.9734/BBJ/2015/12325
Somatic Embryogenesis and Plantlet Regeneration from Protoplast Culture of Stevia rebaudiana In order to develop a high-efficiency and reproducible regeneration protocol for Stevia protoplasts, various factors such as type and concentration of enzymes, osmoticum, incubation time,...
Open access
Research Article10.9734/BBJ/2015/13884
Aim: In the present work, we have studied the effect of L ascorbic acid (LAA) on the regeneration of plants from different families cultured in vitro. Study Design: Plants belonging to three different families are cultured in Murashige and Skoog (MS) medium with and without 1µg/m...
Jurandy Mauro Penitente-Filho, Erly Carrascal, Fabrício Albani Oliveira, Adriana Moreira Zolini, Carlos Tiago Oliveira, Ítalo Augusto Costa Soares & Ciro Alexandre Alves Torres·Biotechnology Journal International·2014
Influence of Dominant Follicle and Corpus luteum on Recovery of Good Quality Oocytes for In vitro Embryo Production in Cattle The quality of oocytes is more critical for the success of bovine in vitro embryo production (IVP) than the conditions of in vitro culture of these struct...
Open access
Research Article10.9734/BBJ/2014/13829