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All published articles 904

Effects of Length of Fermentation and Storage Media on Chemical Properties of Palm Oil

T. A. Ayelegun & P. O. Ajewole · Biotechnology Journal International · 2018

This study examined the effects of different length of fermentation and storage media on quality characteristics of palm oil produced at the processing centre of Department of Agricultural and Bio-Environmental Engineering, The Federal Polytechnic, Ado-Ekiti, Nigeria. Fresh palm...

Open access Research Article 10.9734/BJI/2017/37393

Use of Gene Specific Universal Primers for Isolation of DNA Sequences Encoding Laccase Enzyme from a Wild Isolate of Schizophyllum commune

Vidya Pradeep Kumar, Atul P. Kolte, Arindam Dhali, Chandrashekar Naik & Manpal Sridhar · Biotechnology Journal International · 2017

Laccase enzymes plays a vital role in innumerable biotechnological applications and hence their large scale production has stimulated considerable research. In the present study, degenerate universal primer pairs were employed to isolate laccase gene from a wild isolate of laccas...

Open access Research Article 10.9734/BJI/2017/37022

Comparative Analysis of DNA Extraction Methods in Two Popular Varieties of Finger Millet (Eleusine coracana) from Ethiopia

Aklilu Banjaw Leza, Sunil Tulshiram Hajare & Nitin M. Chauhan · Biotechnology Journal International · 2017

Aims: The aim of this investigation was to standardize the genomic DNA isolation protocol to obtain high quality and quantity DNA for genomic analysis. To accomplish this task, dry leaves of finger millet which are rich in polysaccharides and polyphenols and seeds were utilized a...

Open access Research Article 10.9734/BJI/2017/33145

Prokaryotic Diversity of the Alkaline Lake Acıgöl, Turkey by Using Culture-dependent and Culture-Independent Methods

Gamze Başbülbül, Haci Halil Biyik, Erman Oryaşın & Bülent Bozdoğan · Biotechnology Journal International · 2017

Aims: The prokaryotic diversity of Acıgöl lake, Turkey, was studied in samples taken from soil, water and mud. Culture -dependent and -independent methods were used to analyse the results. Methodology: Bacterial cultures were isolated by using six different growth media. Isolates...

Open access Research Article 10.9734/BJI/2017/36909

Potentialities of Yeast Strains to be Used as Freeze-Drying Starters for the Production of Traditional Sorghum Beer in Côte d’Ivoire

Wahauwouélé Hermann Coulibaly, Fatoumata Camara, Djegba Marie Toka, Koffi Maïzan Jean-Paul Bouatenin, Alfred K. Kouamé, Youan Charles Tra Bi, Anne Abot & Koffi Marcellin Djè · Biotechnology Journal International · 2017

Freeze-drying is a well-known dehydration method widely used to preserve microorganisms. In order to produce freeze-dried yeast starter culture for the brewing purpose of African traditional sorghum beer, we tested tolerance to ethanol stress and evaluated the relative expression...

Open access Research Article 10.9734/BJI/2017/36851

Molecular Markers and Postulation Study of Leaf Rust Resistance Genes in Various Egyptian Wheat Cultivars

Hanaa Abouzied, Eman El Argawy & Walid El-Orabey · Biotechnology Journal International · 2017

The current investigation was carried out at seedling stage under greenhouse condition to: (1) postulate leaf rust resistance genes in ten Egyptian spring wheat cultivars at seedling stage and (2) Verify the presence of resistance genes in the selected cultivars using specific mo...

Open access Research Article 10.9734/BJI/2017/36175

Production and Characterization of Halo Tolerant Alkaline Protease of Enterobacter aerogenes Strain ZB and Its Application

Mohammed A. Almalki, D. S. Deepa Dhas, Rakesh Varghese & Ponnuswamy Vijayaraghavan · Biotechnology Journal International · 2017

Aims: The present investigation was carried out to isolate a halo tolerant Enterobacter aerogenes strain ZB for the production of alkaline protease to degrade protein wastes from the tannery wastewater. Study Design: Halo tolerant protease producing bacteria was isolated from the...

Open access Research Article 10.9734/BJI/2017/37466

In vitro Salinity Evaluation Studies in Golden Berry (Physalis peruviana L.)

Fatma Burcu Celikli, Pınar Akkelle & Ahmet Naci Onus · Biotechnology Journal International · 2017

Aim: Present study, therefore, was conducted in in vitro conditions to study effect of NaCl at varied levels on growth and chlorophyll content of golden berry shoot apices grown in in vitro conditions. Place and Duration: The study was carried out in the Department of Horticultur...

Open access Research Article 10.9734/BJI/2017/37097

Molecular Differentiation of Five Quinoa (Chenopodium quinoa Willd.) Genotypes Using Inter-simple Sequence Repeat (ISSR) Markers

A. M. M. Al-Naggar, R. M. Abd El-Salam, A. E. E. Badran & Mai M. A. El-Moghazi · Biotechnology Journal International · 2017

Knowledge of genetic diversity is one of the important tools used for genetic management of quinoa accessions for plant breeding. This research aimed to molecularly characterize five quinoa genotypes using ISSR markers to reveal genetic polymorphism and identify unique markers fo...

Open access Research Article 10.9734/BJI/2017/37053

Relationship between Neutral Invertase Activity and Sugar Contents in Tomato Fruit and Its Functional Prediction Analysis

Wang Hao-Ran, Yao Jia-Yu, Zhang Tong-Tong, Cui Na, He Sheng, Zhang Rui & Xu Si-Qiong · Biotechnology Journal International · 2017

Aims: Neutral invertase (NI) probably plays an important role in sucrose metabolism of tomato, but main function and mechanism are unclear. In this study, contents of soluble sugar and NI activity were measured at different stages in tomatoes of Solanum chmielewskii and Solanum l...

Open access Research Article 10.9734/BJI/2017/37195

Isolation, Identification and Characterization of Cellulase from a Bacterium Obtained at a Saw-Mill Site in Ile-Ife, Nigeria

A. E. Agbolade, E. F. Aransiola, K. O. Awojobi & M. K. Bakare · Biotechnology Journal International · 2017

Cellulose continues to account for one of earth’s most abundant biomass. Cellulase degrades cellulose, thereby making it one of the most sought after enzyme in the commercial market. This research aimed to characterize cellulase with enviable physicochemical parameters from a bac...

Open access Research Article 10.9734/BJI/2017/37221

Optimization and Lipase Production of Lysinibacillus sphaericus in Domestic Oil Rich Waste Water

Babatunde Aderiye & Adebisi Sulaimon · Biotechnology Journal International · 2017

The present study investigated the growth and lipase production by Lysinibacillus sphaericus in a minimal medium. The growth conditions (i.e oil concentration, nitrogen source, carbon source, metals and non metals) were optimized for maximum production of enzyme. Influence of dif...

Open access Research Article 10.9734/BJI/2017/34290

Inhibitory Effects of 4T1 Breast Tumor Transplantation on Mouse Peripheral Blood Immune Cell Populations

Kexin Zheng, Qilong Li, Chenghao Fu & Shiliang Ma · Biotechnology Journal International · 2017

Aims: One of serious threats to women's health is mammary cancer whose occurrence, development, and treatment are related to the body’s immunological circumstances. In addition, the cancer also imposes the some effects on the body’s immune system. However, the body’s response is...

Open access Research Article 10.9734/BJI/2017/36382

Optimisation of Growth Factors for Effective Use of Phosphate Solubilizing Bacterial Strains and Its Use as Bioinoculants for the Growth of Groundnut (Arachis hypogaea) Plant

Priyanka Anbalagan & Veena Gayathri Krishnaswamy · Biotechnology Journal International · 2017

Phosphorus (P) is one of the essential macronutrients for plant growth. Phosphate solubilizing bacteria (PSB) are organisms which are used as bioinoculants to enhance the plant growth. Plants take phosphate in the form of soluble orthophosphate ions but due to the presence of cal...

Open access Research Article 10.9734/BJI/2017/36243