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All published articles 904

Effects of Surface Sterilization Protocol and Haustorium Removal on Germination and Growth of Hybrid PB121 Coconut Palm (Cocos nucifera L.) Zygotic Embryos Cultured in vitro

Arnaud Agbidinoukoun, Euloge Rimson Somakpe, Serge Sètondji Houédjissin, Florent Engelmann & Corneille Ahanhanzo · Biotechnology Journal International · 2020

Aims: This study aims to identify the best surface sterilization and evaluate the effect of haustorium suppression on in vitro germination of coconut palm (Cocos nucifera L.) zygotic embryos. Study Design: Survival rate and contamination rate of zygotic embryos after different su...

Open access Research Article 10.9734/bji/2020/v24i630122

Recombinant Co-Expression of Collagen A1 (I) Fragment with the Prolyl 4-Hydroxylases (P4H) Subunits in Komagataella phaffii

Esra Avci & Zülal Kesmen · Biotechnology Journal International · 2020

Recombinant collagen and collagen-like products are increasingly replacing animal-sourced collagen that is difficult to produce in safe and standard quality. In this study to produce hydroxylated collagen, a 400 base pair collagen fragment of the bovine COL1A1 gene was co-express...

Open access Research Article 10.9734/bji/2020/v24i630123

RT-PCR Mediated Identification of SARS-CoV-2 Patients from Particular Regions of Bangladesh and the Multi-factorial Analysis Considering Their Pre and Post Infection Health Conditions

Kazi Mahmuda Akter, Tanjum Tushi, Sabrina Jahan Mily, Rashenda Aziz Mohona, Samin Anis, Adittya Kishor Chakraborty, Elmee Tabassum, Tahmid Ul Islam, Ozifatun Jannat Akhi, Israt Sultana Nishe, Badrun Nahar Laxy, Sanjida Sultana Zerin, Abdiqani Jama Roble, Md. Imran Hossain, Sharmin Ahmed & Salauddin Al Azad · Biotechnology Journal International · 2020

Background: SARS-CoV-2 is a type of coronavirus shows bizarre features with fatality rates at 34.4%. The clinical and non-clinical features of COVID-19 might be causative factors for more rapid community spreading compared to MERS and SARS-CoV-1. Objectives: To study the efficacy...

Open access Research Article 10.9734/bji/2020/v24i630121

Effects of Cadmium Stress on Photosynthetic Physiological Characteristics of Two Rorippa Species

Yuan Yun- Ning, Wang Lin- Yu, Chen Hao & Xu Yu- Feng · Biotechnology Journal International · 2020

The photosynthetic physiological characteristics of Rorippa amphilia and Rorippa sylvestris were studied by pot experiment under Cd stress, and 7 Cd treatments were 0, 1, 5, 10, 50, 100, 200(mg·kg-1)respectively. The results showed that, (1) The Chla, chlb, and Ch(la+b) changed...

Open access Research Article 10.9734/bji/2020/v24i630120

Antioxidant, Hypoglycemic and Neuroprotective Activities of Extracts from Fruits Native to the Amazon Region: A Review

Klenicy Kazumy de Lima Yamaguchi & Anderson de Oliveira Souza · Biotechnology Journal International · 2020

The Amazon forest has the largest biome on the planet, and it is estimated that only 16 to 20% of the identified animal, and plant biodiversity. Considering plant diversity, we will highlight the biological properties of the fruits extracts of Arecaceae, Caryocaraceae, Malvaceae,...

Open access Research Article 10.9734/bji/2020/v24i630119

Impact of Drifts Resulting from Pesticide Application on Soil Microorganisms around Waste Receptacles in Port Harcourt City, Nigeria

D. Momoh, C. L. Eze & D. N. Ogbonna · Biotechnology Journal International · 2020

Pesticides are toxic substance used to reduce or kill pests but the deposits on soil environment can remain there for long period of time causing adverse effects on soil microorganisms which are responsible for soil health conditions. This study was carried out to determine the i...

Open access Research Article 10.9734/bji/2020/v24i630118

Structural Characterization of Novel Luciferase from Bioluminescence Fungi Verticillium longisporum

A. A. Yarima, S. M. Sambo, M. D. Kwairanga, K. N. Sharbat, Z. Arifullah & A. Y. Fardami · Biotechnology Journal International · 2020

Luciferase is an enzyme that catalyses a reaction to produce a visible light using an oxidative process, a chemical reaction that is typically referred to as bioluminescent. Insects, bacterial origin or microorganism of marine nature were considered as the mainly sources of disco...

Open access Research Article 10.9734/bji/2020/v24i530117

The Effect of Temperature on Seed Germination and Seedling Growth of Two Invasive Plants in Rorippa

Qiuli Wang, Bo Qu, Juanjuan Mi, Yufeng Xu & Meini Shao · Biotechnology Journal International · 2020

Aims: By studying the response of seed germination and seedling growth of invasive plants, Rorippa amphibia and Rorippa sylvestris, to temperature, the influence of temperature on the invasive ability of two species of Rorippa were further analyzed, which provided a theoretical b...

Open access Research Article 10.9734/bji/2020/v24i530116

Coffee: Constituents and Health Benefits

Shreya Shah & Anil Kumar · Biotechnology Journal International · 2020

Since ancient times, coffee has been one of the widely consumed beverages all over the world. It is renowned for its refreshing, body stimulating effect and unique taste. It belongs to the genus Coffea, class Dicotyledoneae, order Rubiales and family Rubiaceae. Coffee is a natura...

Open access Research Article 10.9734/bji/2020/v24i530115

Assessment of Mutagenicity Induced by Different Mutagens in Coriander (Coriandrum sativum L.)

Prashant N. Kolhe, Mrunalini D. Varne, Sumit N. Deshmukh, Sanjay N. Harke & Sopan G. Wagh · Biotechnology Journal International · 2020

Coriander is one of the most widely used herb. Coriander generally grown for leaf and grain purpose throughout the world. Induction of mutation is considered to be effective in improving various characteristics in plants like seed germination, plant vigour, yield etc. In present...

Open access Research Article 10.9734/bji/2020/v24i530114

In vitro Callogenesis and Organogenesis from Carolina Reaper (Syn. Capsicum Chinense Jacq.) and Chromosomal Analysis

Bruno Henrique Gomes, Fabrícia de Matos Oliveira, Ana Paula Oliveira Nogueira & Robson Jose de Oliveira Júnior · Biotechnology Journal International · 2020

Aims: The aim of this paper is to develop an in vitro organogenesis and callogenesis protocol for Carolina Reaper pepper, and to determine the karyotype and nucleoli of this cultivar. Methodology: The MS medium with supplemented with indole-3-butyric (0, 1, 2 and 4 mg L-1) and ki...

Open access Research Article 10.9734/bji/2020/v24i530113

A Study on Keratin-Associated Protein (KAP) 3.2 Gene and Its Polymorphism in Sandyno Breed of Sheep

R. Bharathesree, R. Saravanan, M. Jeyakumar & N. Murali · Biotechnology Journal International · 2020

The current study investigates the polymorphic patterns of keratin-associated protein (KAP) 3.2 gene in Sandyno breed of sheep. Genomic DNA was isolated from blood samples of 51 numbers of Sandyno breed. Ovine specific primer associated PCR amplification of KAP 3.2 gene revealed...

Open access Research Article 10.9734/bji/2020/v24i430112

In vitro Propagation of Bacopa monnieri (L.)

Poornima Raj, J. Anbumalarmathi & S. Aruna Sharmili · Biotechnology Journal International · 2020

An experiment was conducted for standardization of in vitro propagation technique of Bacopa monnieri (L.), a medicinal herb of India. Healthy leaf segments of the herb were used as explants with basic Murashige and Skoog (MS) medium containing various combinations of different gr...

Open access Research Article 10.9734/bji/2020/v24i430111

Exo-Inulinase Production by a Catabolite Repression-Resistant Mutant Thermophilic Aspergillus tamarii-U4 in Solid State Fermentation

E. O. Garuba & A. A. Onilude · Biotechnology Journal International · 2020

In this study, spores of inulinase-producing thermophilic Aspergillus tamarii were subjected to UV mutagenesis, and colonies obtained were screened for inulinase production on inulin-glucose agar. The thermal stability of the inulinase was also investigated. A mutant strain U4 wa...

Open access Research Article 10.9734/bji/2020/v24i430110

Technical Sheet of the Preparation of Traditional Cassava Starters Used for Attieke Production in Côte d'Ivoire

Boli Zamblé Bi Irié Abel, Bouatenin Koffi Maïzan Jean-Paul, Kouamé Kohi Alfred, Coulibaly Wahauwouele Hermann, Kakou Abodjo Celah, Rose Koffi- Nevry & Djè Koffi Marcellin · Biotechnology Journal International · 2020

Aims: The aim of this study was to identify the different methods of preparing the traditional cassava starters used for attiéké production in Côte d'Ivoire, allowed the recounting of five different production methods. Study Design: Sampling of cassava starters were collected fro...

Open access Research Article 10.9734/bji/2020/v24i430108