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All published articles 904

Effects of Process Parameters on the Activities of Enzymes by Different Species of Aspergillus in Crude Polluted Soil Sites

J. M. Madu, A. I. Ogbonna & C. I. C. Ogbonna · Biotechnology Journal International · 2021

The effects of temperature, pH and incubation time on amylase, protease and cellulase activity by different species of Aspergillus in crude oil polluted soil sites in Nigeria were studied. Incubation period, Optimal pH values and temperatures for the enzymes produced by the diffe...

Open access Research Article 10.9734/bji/2021/v25i330140

Isolation and Identification of Resistant Bacteria from Petroleum Producing Vicinity by Gene Amplification and Sequencing

O. Aleruchi & O. Obire · Biotechnology Journal International · 2021

This investigation focuses on molecular identification of antibiotic resistant bacteria isolated from petroleum producing vicinity using 16S rRNA sequencing based technique. The bacterial 16s rRNA gene sequences were amplified using polymerase chain reaction, sequenced,  characte...

Open access Research Article 10.9734/bji/2021/v25i330139

Cellulase: Distribution, Production, Characterization and Industrial Applications

Pratibha Maravi & Anil Kumar · Biotechnology Journal International · 2021

Cellulase enzyme complex is comprised of three enzymes namely exo-glucanase, endo-glucanase and β-glucosidase which act synergistically to deconstruct cellulosic biomass in order to produce fermentable sugars. The enzymes are produced naturally by the living organisms such as bac...

Open access Research Article 10.9734/bji/2021/v25i330143

Effect of Pre and Post Infection Administration of Zinc and Selenium on some Biochemical indices in Wistar Rats infected with Trypanosoma brucei

Timothy Tese, Kola Matthew Anigo, Alice Onyeche Jonah, Meshack Anthony Kugama, Tinok Andrew, Janet Larai Ibrahim, Raymond Arumona & William Denbon Genesis Chinten · Biotechnology Journal International · 2021

Aims: The effect of pre and post-infection administration of zinc and selenium on Trypanosoma brucei brucei infection in wistar rats on some biochemical parameters were investigated. Study Design:  The study was designed to evaluate the effect of pre and post infection administra...

Open access Research Article 10.9734/bji/2021/v25i230138

Genetic Characterization of Domestic Apple Varieties from Tuzla Canton, BiH

Ensar Salkić, Besim Salkić, Amela Hercegovac, Aldijana Avdić, Azra Dorić & Emir Imširović · Biotechnology Journal International · 2021

Aims: The basic precondition for apple breeding is the genetic diversity of varieties, which implies a large number of different, positive genes that enable adaptation to different weather conditions, resistance to new diseases and pests. One of the reliable sources of genetic di...

Open access Research Article 10.9734/bji/2021/v25i230137

Optimization of Selected Process Parameters Affecting Yield of Green Synthesized Silver Nanoparticles and Their Antibacterial Activity

F. A. Ekaji, C. O. Akujobi & S. I. Umeh · Biotechnology Journal International · 2021

Aims: To optimize effects of selected process parameters affecting yield of green synthesized silver nanoparticles and their antibacterial activity. Study Design: Study was designed with 3 factors Box Behnken Design (Minitab 17) and Response optimizer (Minitab 17) was used to det...

Open access Research Article 10.9734/bji/2021/v25i230136

Construction of an Agrobacterium Mediated RNAI Genetic Transformation Vector Targeting the Replicase Gene of Indian cassava mosaic virus and Evaluation of Their Transformation Ability in Cassava Immature Leaf Lobes

T. Anuradha & P. Prabha · Biotechnology Journal International · 2021

Cassava mosaic virus is one of the major problems affecting cassava industry in India. Currently there are no effective strategy to completely protect cassava from cassava mosaic viruses. In order to attain cassava mosaic virus resistance RNAi vectors targeting the replicase gene...

Open access Research Article 10.9734/bji/2021/v25i230135

Molecular Detection and Typing of Cervical Human Papillomavirus in a Selected Population in Bayelsa State: Comparison with the National and International Trend and Clinical Significance

Y. I. Oboma, A. A. Ngokere, Y. M. Tatfeng, S. I. Musa, I. A. Ibrahim, S. E. Itodo & A. B. Abdu · Biotechnology Journal International · 2021

Background: The link between cervical lesions and human Papillomavirus (HPV) 16 and 18 is well established, but the magnitude of the risk of association and the importance of other high-risk hpv types is uncertain in Bayelsa state. Aims: The study was aimed at detecting and typin...

Open access Research Article 10.9734/bji/2021/v25i230134

Biochemical Characterization of Two Polygalacturonases Purified from the Digestive Juice of the Snail Limicolaria flammea

Armand Kouassi Kanga, Djary Michel Koffi, Bernard Téhi Sea, Jaures Oscar Gbotognon & Lucien Patrice Kouamé · Biotechnology Journal International · 2021

Polygalacturonases constitute the major part of pectinase preparations for many bioprocess purposes. Investigation on the digestive juice of snail Limicolaria flammea led to purification of two polygalacturonases named PG1 and PG2. Properties of these enzymes were examined to exp...

Open access Research Article 10.9734/bji/2021/v25i130133

Piranhea trifoliata an Amazonian Plant with Therapeutic Action: A Review

Lucineide Teixeira Vieira, Márcia Pinheiro da Silva, José Dobles Dias dos Reis Júnior, Carlos Antônio Couto Lima & Anderson Oliveira Souza · Biotechnology Journal International · 2021

The use of medicinal plants reflects the reality of a part of human history. The Brazilian population with limited access to public health programs led to the development and conservation of ethnobotanical knowledge-rich information regarding medicinal plants. However, popular wi...

Open access Research Article 10.9734/bji/2021/v25i130132

Phytochemical Evaluation and Antioxidant Capacity of Ganoderma lucidum and Pleurotus pulmonarius in Ibadan, Nigeria

G. Aruwa, C. O. Adenipekun, S. T. Ogunbanwo & E. O. Akinbode · Biotechnology Journal International · 2021

Aims: To evaluate the phytochemical constituents and antioxidant properties of two selected mushrooms in Ibadan, Nigeria. Study Design: An experimental and descriptive study was conducted using the fruit bodies of Ganoderma lucidum collected from the Botanical garden University o...

Open access Research Article 10.9734/bji/2021/v25i130131

Microbial Diversity of Nigerian Sludge and Its Potential for Use as Biofertilizer

Opeyemi Fatunla, Joseph Essien, Utibe Ofon, Solomon Shaibu & Emmanuel Dan · Biotechnology Journal International · 2021

Sludge samples were collected from a wastewater treatment plant in Nigeria for characterization and evaluation for agricultural applications. Conventional and Molecular techniques were adopted for the isolation and identification of indigenous microorganisms and resulting isolate...

Open access Research Article 10.9734/bji/2021/v25i130130

In vitro Evaluation of the Fungicidal Potential of Aqueous and Methanolic Extracts of Thevetia peruviana on the Development of Rigidoporus lignosus, Causal Agent of White Root rot of Hevea brasiliensis

Serge Bertrand Mboussi, Alain Heu, Abdou Nourou Kone Nsangou, Martial Douanla Ajebe, Godswill Ntsomboh Ntsefong, Jules Patrice Ngoh Dooh & Zachée Ambang · Biotechnology Journal International · 2021

Aims: The objective of this work is to evaluate the effect of (aqueous (EAq) and methanolic (ME)) extracts of Thevetia peruviana on the in vitro development of Rigidoporus lignosus. Study Design: A synthetic fungicide (Onazol 100), two extracts at differents concentrations: C1 =...

Open access Research Article 10.9734/bji/2021/v25i130127

Selection and Validation of Reference Genes for Quantitative Real-Time PCR Expression Analysis of Candidate Genes in Carposina sasakii (Lepidoptera: Carposinidae)

Zuobing Yan, Yongli Li, Zhou Zhou, Yongan Zhang & Liangjian Qu · Biotechnology Journal International · 2020

Carposina sasakii is one of the most important pests on the quality of stone and pome fruits. Investigation of a gene expression level in the species is hampered because of the gap of validated reference genes. The expression variation in the transcription levels of eight candida...

Open access Research Article 10.9734/bji/2020/v24i630124

Distribution, Importance and Diseases of Soybean and Common Bean: A Review

Gaurav Singh, Garima Dukariya & Anil Kumar · Biotechnology Journal International · 2020

The crops belonging to the family Leguminosae are the second to cereal crops of agricultural significance due to their high nutritional value. Soybean is native to East Asia and was originated in China while common bean is native to Central and Southern America originated in Peru...

Open access Research Article 10.9734/bji/2020/v24i630125