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All published articles 904

Unlocking Nature’s Secret: Microbiological and Nutrient Profiling of Fermented Apple

Omodara, Tolani Rachael, Ajayi, Olayemi Elizabeth & Kotila, Oluwasefunmi Omolabake · Biotechnology Journal International · 2024

Aim: This study aimed to investigate the microbiological and nutrient composition of naturally fermented apple cider.   Place and Study Duration: Department of Microbiology, between June and August 2023. Methodology: The purchased apples were weighed, cut into dice, cleaned, and...

Open access Research Article 10.9734/bji/2024/v28i2714

Exploring Gene Therapy Strategies for Cystic Fibrosis: A Comprehensive Review of CFTR Gene Mutations, Bioinformatics Analysis, and Emerging Therapeutic Approaches

Hassan S. Darwish, Hajer Almamri, Manohar Noone, Azher Alhabsi & Una Gibbons · Biotechnology Journal International · 2024

Aim: This study aimed to identify the most promising gene therapy approach for treating patients with cystic fibrosis. Background: Cystic fibrosis is a rare autosomal recessive disorder affecting malabsorption, malnutrition, and lung function. It results from genetic mutations in...

Open access Research Article 10.9734/bji/2024/v28i1713

Biochemical Analysis and Characterization of Silver Nanoparticles Synthesized by the Endophytic Fungus Plectosphaerella oligotrophica S8A26 Associated with Anaphalis contorta

Kistu Singh Nongthombam, Shyamkesho Singh Mutum & Radha Raman Pandey · Biotechnology Journal International · 2024

Endophytic fungi associated with medicinal plants produce compounds that have vast biological activities. Anaphalis contorta is a highly therapeutic plant that has been used in traditional medicine by various communities. In the present study, the endophytic fungus Plectosphaerel...

Open access Research Article 10.9734/bji/2024/v28i1711

Biomolecular Characterization of Lactic Acid Bacteria (Lactobacilli and Cocci) Isolated from Nonnonkoumou, a Artisanal Milk Curd in Daloa, Côte D’ivoire

Dogoua Koko Guillaine Jostasie, Assohoun-Djeni Nanouman Marina Christelle, Kouassi Kouassi Clément & Djeni N’dédé Théodore · Biotechnology Journal International · 2024

Nonnonkoumou is an artisanal sour milk consumed in Côte d'Ivoire, particularly in the town of Daloa. Its production is carried out under uncontrolled conditions and uses a variety of lactic acid bacteria. The main objective of this study was to characterize the cocci and lactobac...

Open access Research Article 10.9734/bji/2024/v28i1710

A New Era for Gold Nitrate Nanoparticle Applications

I. T. D. Botelho, S. R. B. Ferreira & W. S. Ferreira · Biotechnology Journal International · 2024

Gold nanoparticles are included in the group of metallic nanoparticles, and their application has become possible recently because of new optical equipment, electronic devices, and probes capable of manufacturing, detecting, and identifying biomolecules of medical interest. Among...

Open access Research Article 10.9734/bji/2024/v28i1708

Protecting Genetic Genealogical Databases from Identical-by-State Probing Attacks: A Machine Learning-Based Approach

Enow T. A. A. & Lum A. F. · Biotechnology Journal International · 2023

Identical-by-state (IBS) probing is a way of attacking a public genealogy database to discover the identities of people who share specific qualities. The attacker creates an IBS-inert DNA sequence (IBS-inert-DNA-sequence) and combines it with a sequence containing the trait of in...

Open access Research Article 10.9734/bji/2023/v27i6707

Genetic Diversity and Phylogenetic Relationships among Jatropha curcas L. Genotypes from Eastern India

Sanjay Singh & Priyamvada Singh · Biotechnology Journal International · 2023

Genetic diversity and relationships among 31 genotypes collected from wide geographical range in eastern India was studied employing Random Amplified Polymorphic DNA (RAPD) markers. 19 markers (identified out of 25) produced 139 band in total. Off these 139 bands, 131 bands were...

Open access Research Article 10.9734/bji/2023/v27i6706

Anthelmintic Potential of Vernonia amygdalina on Toxocara canis in Domesticated Dogs

Adams, Eka & Ekott, Emmanuel · Biotechnology Journal International · 2023

Toxocariasis is a worldwide zoonotic parasitic infection and one route of preventing the disease in dogs and subsequent transmission to humans, especially children, is through treatment with anthelmintic. A study aimed at assessing the anthelmintic efficacy of Vernonia amygdalina...

Open access Research Article 10.9734/bji/2023/v27i6705

Effects of Different Temperature Treatments on Seed Germination and Seedling Growth of Rorippa sylvestris

Hai ling Yan, Gui Li Shi, Wen Peng Shi, Mei Ting Jin, Yi Nuo Sun, Bo Qu & Mei Ni Shao · Biotechnology Journal International · 2023

Temperature is one of the important conditions for plant growth, and an important factor affecting the establishment and spread of invasive plants. In this study, the northern perennial invasive plant (Rorippa sylvestris) was taken as an example. By comparing the seed germination...

Open access Research Article 10.9734/bji/2023/v27i6704

Precipitation Using Organic Solvents for Purifying Lipase from Preussia africana

Eric E. A. Ferreira, Brenda C. Pereira & Daniela B. Hirata · Biotechnology Journal International · 2023

Aims: The present study aimed to purify a new lipase from the endophytic fungus Preussia africana (P. africana). Study Design: Lipases were produced through fermentation and precipitation using different organic solvents and characterized at optimal temperature and pH conditions....

Open access Research Article 10.9734/bji/2023/v27i6702

Prevalence of Enterobacteria Species in Different Hospital Wards of a Tertiary Health Facility in Imo State, Nigeria

Emmanuel Chimela Ibe, Ijeoma Emeka-Nwabunnia & Reuben Nwoye Okechi · Biotechnology Journal International · 2023

Aim: to assess the prevalence of Enterobacteria species in different hospital wards in a healthcare outfit in Imo State, Nigeria. Study Design: The randomized complete block sampling design was adopted for the study. Place and Duration of the Study: Sample: Imo State University T...

Open access Research Article 10.9734/bji/2023/v27i6703

Virulence and Molecular Detection of Cassava Mosaic and Brown Streak Virus Genes on Elite Mutant Cassava Lines

Lydia Chepkoech Kimno, Javan Omondi Were, Isaac Kofi Bimpong, Miriam Gaceri Kinyua & Stephen Kimno · Biotechnology Journal International · 2023

Unavailability of germplasm with Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) tolerance genes remains the main bottleneck towards management of the two diseases in tropic and sub-tropic regions in Africa. We assessed the pathogenicity of two viruses and es...

Open access Research Article 10.9734/bji/2023/v27i6701

Comparative Effect of Spent Mushroom Compost and NPK Fertilizer on the Growth of Sorghum bicolor L. Moench and Zea mays L. Using Fodder Hydroponics Techniques

Oluwabamiro K. S., Adenipekun C. O., Ijimbili S. B. & Oyetunji O. J. · Biotechnology Journal International · 2023

The grains of maize and sorghum were converted to grass in the screenhouse for eight days. The treatments evaluated were control (water), spent mushroom compost (SMC), NPK fertilizer, and SN (combination of SMC and NPK). Each treatment was replicated thrice in a complete randomiz...

Open access Research Article 10.9734/bji/2023/v27i5700

Estimation of the Electrical Energy Potential of Rice Husk Biomass Generated within Ebonyi State, South Eastern Nigeria

Igbo, Michael Elem, Nnabuchi, Mishark. Nnamdi, Igbo, Nkechinyere Elem, Nwodo, Emeka & Ogbonna, Pius · Biotechnology Journal International · 2023

Nigeria’s energy sector has been ailing for the past two decades due to its excessive dependency on petroleum and lack of diversity in energy sources in the energy supply mix. To address this huge energy gap, biomass conversion power plant (BCPP) is considered as a “bridging solu...

Open access Research Article 10.9734/bji/2023/v27i5699