Isolation and Screening of Agriculturally Important Bacteria (PGPR) from Organic Sources of Nutrient (Panchgavya, Jeevamrit and Farm Yard Manure) for Future Use

Abstract

Agriculture is an essential component to maintain the human health but due to sudden increase in population the demand has increased and to meet the requirement the use of chemical fertilizer has also increase. The continuous use of chemical fertilizer and pesticides degrade the quality and fertility of soil and also reduce the yield of various crops. The organic sources such as panchgavya, jeevamrit and Farm Yard Manure consists load of beneficial microorganisms that promote the plant growth, yield and promote natural resources. The present study was carried out by isolating the bacteria from organic sources (panchgavya, jeevamrit and FYM) because minute amount of bacterial culture is suffient to prepare large one according to need and can be stored for longer time as compare to organic input. The samples of panchgavya, jeevamrit and FYM were collected from different location of Himachal Pradesh. A total of 32 bacterial isolates from panchgavya, 43 from jeevamrit and 59 from FYM were isolated and screened for multiple plant growth promoting traits. Only 4 bacterial isolates from panchgavya, 2 from jeevamrit and 2 from FYM were preferred on the basis of maximum plant growth promoting traits like P-solubilization, Nitrogen fixer, Siderophore production, HCN production, IAA production and Antifungal activity. From the selected bacterial isolates 67.78% were P- solubilizers, 60.37% were Nitrogen fixer, 62.37% were Siderophore producer, 34.29% HCN producer, 45.88% IAA producer and 78.22% show antagonism against Pythium graminicolum and 74.81% show antagonism against Collectotrichum capsici. Hence these bacterial isolates have potential to act as biofertilizer for environment friendly sustainable agriculture systems and alternative to harmful chemicals.