Immunogenicity of Bacterial Protoplasmic Sonicate Proteins

Abstract

Bacterial Protoplasmic Sonicate Proteins BPSP were recovered from skin burn Pseudomonas aeruginosa and Klebsiella oxytoca infections. These proteins   were considerd as immunogens in rabbits. Mucosal and systemic, humoral and cellular immune immune responses were matched. The P. aeruginosa protoplasmic sonicate proteins induces an increase in; NBT % neutorphil phagocytosis, significant leukocyte migration inhibition cytokines, IL6 cytokine elevation and rise up of humoral agglutinins responses. While, K. oxytoca protoplasmic sonicate proteins induces increase of; humoral agglutinins and IL6 cytokine as well as non-signifcant increase in leukocyte inhibitory cytokines. Both at mucosal and systemic compartments. The study highlights the potentials of these proteins as candidates for developing vaccines against multidrug resistance infections, particularly in burn patients. These findings underscore the importance of immunogenicity in advancing bacterin development in clinical use.