Phytochemical Profiling and Evaluation of Antioxidant and Anti-inflammatory Activities In vitro of Newbouldia laevis and Flueggea virosa Leaf Extracts

Abstract

Traditional medicine use has surged over the past 20 years, with many people using it to treat various health issues. Ethnobotanical and pharmaceutical surveys revealed their application in many areas. Ethnobotanical and pharmaceutical surveys have revealed their application in many areas. Analyzing the antioxidant and anti-inflammatory properties and quantifying the polyphenol, flavonoid, and tannin contents of the methanolic and hydroethanolic extracts from the leaves of Flueggea virosa and Newbouldia laevis.is the goal of this study. All extracts exhibited notable antioxidant activity. Among them, we noted that the hydroethanolic extract of F. virosa stood out by displaying the highest antioxidant performance, with values of 3.30 ± 0.05; 7.81 ± 0.54 and 18.72 ± 0.05 mMol AAE/mg according to the ABTS, APM, and DPPH methods, respectively. The quantitative analysis of phenolic compounds showed that extracts from F. virosa leaves were so rich in polyphenols (14328 and 22512 μg EAG/mg extract). From the study of anti-inflammatory activity, it was found that a good activity was obtained for all extracts in particular the hydroethanolic extract of N. laevis (IC50 = 0.87 ± 0.02 mg/mL). From these results, it is deduced that the content of phenolic compounds is very high for F. virosa and N. laevis in contrast to flavonoids and tannins that give them these anti-inflammatory and antioxidant powers. The study supports the use of these plants in traditional medicine.