Gas Chromatographic FID Evaluation of Roots of Manniophyton fulvum

Abstract

The phytochemical composition of the root extract of Manniophyton fulvum was screened by gas chromatography coupled to flame ionization and pulse flame photometric detectors. Among the twenty flavonoids detected, only quercetin (107.44mg/100g), kaemoferol (66.19mg/100g), quercetrin (49.87mg/100g), rutin (28.52mg/100g) and, apigenin (28.67mg/100g) representing (37.99%,23.41%, 10.14%, 17.64%, and 10.09%) respectively were prominent. The rest were in insignificant amount. The aqueous root extract contain nine glycoside compound consisting mainly of digoxin (27.166mg/100mg 58.5%), digitoxin (17.9 mg/100g (38.51%) and 0.8mg/100g (2.99%) of kampferol-3-rhamnoside.The rest were of a very little amount. For saponin, the FID gas chromatography analysis revealed that euphol, saponine, sapogenin had the highest concentration of 20.268490mg/100g, 0.3369mg/100g, and 0.065978mg/100g making up of 97.9%,1.62% and 0.3%  percentage composition respectively. The rest were in insignificant amount. Seven benzoic acids were detected by the GC- FID consisting mainly of p-hydroxyl benzoic 6.288160mg/100g (65.4%), gallic acid 2.96280 mg/100g (30.8%) and gentisic acid 0.355mg/100g (3.71%). Out of the ten carotenoid detected, the highest concentration were violaxanthine 47.80526 (49.95%), astraxanthine 26.62538, (27.82%), lutein 8.24911 (8.93%) and neoxanthine 6.54195mg/100g. (6.84%). For phytosterol, the bioactive compound include sitosterol 14.2007mg/100g (71.53%), campesterol  3.54mg/100g (17.85%) and stigmasterol 2.1093mg/100g (10.6%) making up highest concentration. Three allicin compound were detected which include diallylthiosulphinate (95.92%), methyl allylthiosulphinate (3.50%) and allyl methyl thiosulphinate (0.58%). These results show that Manniophyton fulvum has many bioactive molecules suggesting strong therapeutic and nutraceutical potentials suggesting their likely uses in the management and prevention of diseases.