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Research Article Open access CC BY 4.0

Validation of the Method for Determination of Melamine and Investigation its Trace in Milk from Vietnam by LC-MS/MS

Quang Hieu-Tran

Asian Journal of Applied Chemistry Research · pp. 13–19 · Published 24 Feb 2021

10.9734/ajacr/2021/v8i130182

Abstract

This work describes a rapid, selective, and sensitive method by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect melamine (MEL) in milk and dairy products. The optimal conditions of liquid chromatographic separation extraction and mass spectroscopy of MEL have also been examined. The linear range for analyte detected by the method was 0.5÷100.0 ng/mL, with correlation coefficients was 0.999.  Mean recoveries of the method in the real samples at three spike levels (low, medium, and high) were within the range of 98.5% ÷102.5% (n =7). LOD, LOQ values of the method were 10 and 30 ng/mL, respectively. The influence of the matrix effect on the accuracy, repeatability, and recovery of the process was insignificant. The proposed method was used to quantify the content of this compound in various real samples, which were collected in Ho Chi Minh City-Vietnam in 2020.

LC-MS/MS tandem melamine matrix effect.

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