Studies on the Detection of Viruses Infecting Capsicum Species in the Northern Zone of Cross River State, Nigeria

Abstract

The objective of this study was to determine the occurrence, detection, and geographical spread of viruses that infect pepper (Capsicum spp.) in six towns spanning two local government areas (Yala and Ogoja) in the northern zone of Cross River State. A total of 348 leaf samples from symptomatic and asymptomatic pepper plants were randomly collected from farmer's fields during a survey in 2023–2024. The samples were then preliminary tested using an antigen coated plate enzyme-linked immunosorbent assay (ACP-ELISA) with seven antibodies specific for ToLCV, Cowpea aphid borne mosaic virus (CABMV), Cucumber mosaic virus (CMV), Potato virus X (PVX), Tobacco mosaic virus (TMV), and Pepper veinal mottle virus (PVMV). The findings showed that the viruses that infect pepper in Yala had a high cumulative mean incidence, accounting for 56.9% (198 infected samples). Yache in Yala had the highest virus incidence at 90%, while Bansara in Ogoja had the lowest at 40%. At different rates, ToLCV, CMV, PVY, and PVMV were found in the samples; PVX, TMV, and CABMV were not found. The CMV virus was the most commonly discovered of them. Additionally, mixed infections between the viruses—between ToLCV and PVY in Yache and between CMV and PVMV in Bansara—were found. This is the first report on account of viruses found in pepper in the northern part of Cross River State. The findings show that the viruses that cause the infections are common and might potentially be a threat to the production of pepper in that region.