Isolation and Purification of an Antifungal Protein from Kiwi Fruits and Demonstration of Its  Antifungal Activity

Abhishek Walia; Neerja Rana; Vishal Rana

doi:10.9734/JAMB/2017/32671

Research Article Open access CC BY 4.0

Isolation and Purification of an Antifungal Protein from Kiwi Fruits and Demonstration of Its Antifungal Activity

Abhishek Walia, Neerja Rana, Vishal Rana

Journal of Advances in Microbiology · pp. 1–7 · Published 27 Apr 2017

10.9734/JAMB/2017/32671

Abstract

A single polypeptide low molecular weight 25 KDa thaumatin-like antifungal protein was purified from Hayward variety of kiwifruits. It was purified to apparent homogeneity involving saline extraction, (NH4)2SO4 precipitation, anion exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-100, respectively. In present work, simple chromatography procedure was described for purifying antifungal activity form the pulp of Hayward kiwifruit. It furnishes evidence of existence of antifungal activity against Fusarium oxysporum (83.72%) and Rhizoctonia solani (33.78%) and did not show any inhibitory effect against Dematophora necatrix. The proteins have low molecular weight and would be potential importance for increasing disease resistance in plants through genetic engineering and can be used as bio control for controlling fungal diseases in plants.

Antifungal protein kiwifruits Actinidia chinensis purification pathogenesis related proteins

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Abhishek Walia, Neerja Rana, Vishal Rana (2017). Isolation and Purification of an Antifungal Protein from Kiwi Fruits and Demonstration of Its Antifungal Activity. Journal of Advances in Microbiology , 1–7. https://doi.org/10.9734/JAMB/2017/32671

Affiliations

Abhishek Walia — Department of Microbiology, DAV University, Jalandhar, Punjab – 144012, India
Neerja Rana — Department of Basic Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan – 173230 (H.P.), India
Vishal Rana — Department of Fruit Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan – 173230 (H.P.), India

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