Ex-vivo Acetylcholinesterase and Butyrylcholinesterase Inhibitory Activities Assay of G. asiatica and G. tiliaefolia (Tiliaceae) Leaves
Md. Kamrul Islam Joy, Nazma Akhter, Rawnak Reza, Mst. Sayma Rahman Antara, Md. Shariful Islam, Celia-Vargas de la Cruz, Md. Rakibul Islam
Annual Research & Review in Biology · pp. 1–10 · Published 21 Jan 2020
10.9734/arrb/2019/v34i230149Abstract
Aims: Our study was carried out to appraise acetylcholinestrase (AChE) and butyrylcholinestrase (BChE) inhibitory activities of Grewia asiatica and Grewia tiliaefolia leaves extracts. Study Design: For the purpose of these experiments the extracts were subjected to an ex-vivo study. Place and Duration of Study: The study was carried out between June 2018 to December 2018 in the Department of Pharmacy, Southeast University, Dhaka, Bangladesh. Methodology: In this study, cholinesterase inhibitory activities of different fractions of crude ethanol extract of both plants were examined using swiss albino mice at 300 mg/kg b.w. dose. We determined anti-acetylcholinestrase (AChE) and anti-butyrylcholinestrase (BChE) activities using slightly modified Elman coupled enzyme assay. Results: The highest inhibition of bovine brain acetylcholinesterase and human blood butyrylcholinesterase were exhibited by PEF and CLF of G. asiatica with the IC50 values were found to be 55.88 μg/ml and 26.14 μg/ml respectively whereas the highest inhibition of bovine brain acetylcholinesterase and human blood butyrylcholinesterase were exhibited by CLF of G. tiliaefolia. Conclusion: The result of the present study on various fractions of these plants has a considerable anti-acetylcholinesterase and anti-butyrylcholinesterase activities which suggest its effectiveness against various neurodegenerative disorders.
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