Endophytic Microorganisms in Leaves of Moringa oleifera Collected in Three Localities at Pernambuco State, Northeastern Brazil
Igor F. A. C. Souza, Thiago H. Napoleão, Kêsia X. R. F. de Sena, Patrícia M. G. Paiva, Janete M. de Araújo, Luana C. B. B. Coelho
Microbiology Research Journal International · pp. 1–7 · Published 16 Mar 2016
10.9734/BMRJ/2016/24722Abstract
Aims: This work investigated the population density of endophytic microorganisms from Moringa oleifera leaves collected in three localities at the state of Pernambuco (northeastern Brazil): Urban (campus from the Federal University of Pernambuco, UFPE) and forest (botanical garden) areas at Recife city and an urban area (industrial district) at Caruaru city. Place and Duration of Study: Department of Antibiotics and Department of Biochemistry from Federal University of Pernambuco, between July 2014 and July 2015. Methodology: Sodium hypochlorite was used to disinfect the leaves, which were macerated in PBS buffer and separately sowed on seven culture media containing antibacterial or antifungal agents. Results: The most of endophytes isolated were bacteria and the highest density was found in leaves from the forest area. With respect to the fungi, there was no statistical difference between the density in leaves from UFPE campus and the botanical garden while no fungal isolates was obtained from leaves collected in Caruaru. The highest diversity of endophytes was found in the leaves from the botanical garden, with 111 different isolates. A total of 71, 94 and 50 bacterial isolates were obtained from leaves of UFPE campus, botanical garden and Caruaru, respectively. The number of fungal isolates were 17 (UFPE campus) and 12 (botanical garden). Conclusion: In conclusion, the methodology employed in this work was effective for the isolation of endophytes; climatic and geographical conditions may interfere in density and diversity of endophytes from M. oleifera leaves.
Cited by 12
Manal F. Hemeda, Zeinab M. H. Kheiralla, Hoda H. Abo-Ghalia · Journal of Scientific Research in Science · 2022
S. Khaekhum, Jindarat Ekprasert, Thanapat Suebrasri · 2021
Wilmer Giovanny Mosquera, Libeth Yajaira Criado, B. Guerra · Biomédica: revista del Instituto Nacional de Salud · 2020
Zuhura Mwanga, E. Mvungi, Donatha D. Tibuhwa · Tanzania Journal of Science · 2019
Hortência Farias de Andrade, L. C. D. De Araújo, B. D. dos Santos · Brazilian Journal of Microbiology · 2018
Saranya Shankar, Gayathri Segaran, Mythili Sathiavelu · Biocontrol Mechanisms of Endophytic Microorganisms · 2022
Ekpunobi Nzube F, Enyi Emmanuel O · Journal of Microbiology & Experimentation · 2022
Bartholomew Adeleke, Ayomide Fadiji, Modupe Ayilara · Horticulturae · 2022
Bonface M. Nthuku, Esther W. Kahariri, Johnson K. Kinyua · Microbiology Research · 2023
Related research
- Current Status and Case Studies on the Role of Endophytes in Insect Pest Management: A Review — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
12
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.