Biosynthesis of Aloe Vera Gel with Zinc Oxide Nanoparticles (Zno-Nps) for Burn Wound Treatment in Rabbit Model

Abstract

To see the efficacy of combined preparation of aloe vera gel and Zinc Oxide nanoparticles (ZnO NPs) on burn wound treatment has been studied in Rabbit model. Sixteen male New Zealand White male rabbits were divided into four different groups and data were collected on healing rate, healing time and WBC count in artificial hot iron burned rabbit model. Group T0, T1, T2 and T3 were as control, aloe vera gel, aloe vera gel with ZnO nanoparticles and Bactrocin® respectively. Area of the wound and healing time was determined at weekly interval. WBC was enumerated weekly interval using a hematology analyzer. The experimental results showed that rate was significantly increase in T2 (94.75±1.31) group, better by T3 treated group (92.50±1.32), good by T1 (85.50±2.21) and worst by T0 (70.50±2.72). The White Blood Count (WBC) count as greatly increased in the non-treated group in most of the cases (Day 0 and Day 28), but the best WBC count was found in (Bactrocin®) in the rest of the cases (9300±37.50, 8500±37.36 and 6600±24.25 cells/µl for 1st week, 2nd week and 3rd week respectively), while the good count was obtained from T2 (Aloe vera gel with ZnO nanoparticles) treatment. The overall result of the study showed that the use of aloe vera gel with ZnO nanoparticles has given faster wound healing with minimum days requirement, which proved that without antibiotics, wound healing was also possible by using of biosynthetic ZnO nanoparticles and can be recommended for burn wounds healing treatment in rabbits.