Effects of the Combined Treatment of Ethanol Extract of Moringa oleifera and Pleurotus ostreatus on Blood Glucose, Amylase and Lipid Profile of Alloxan-induced Diabetic Wistar Albino Rats

Abstract

Objective: To investigate the effect of the combined treatment of Moringa oleifera and               Pleurotus ostreatus on blood glucose, amylase and lipid profile of diabetic rats. Study Design: Animal experimental study. Place of Study: Department of Biochemistry, Faculty of Science University of Port Harcourt P.M.B 5323 Port Harcourt Nigeria. Year of Analysis: 2015. Methods: Diabetes mellitus type 1 was induced to the animals intraperitoneally with 120 mg/kg body weight of alloxan. The combined ethanol extract was administered to the diabetic wistar albino rats at a different combination percentage of 60% (1,800 mg/kg) Moringa oleifera and 40% (600 mg/kg) Pleurotus ostreatus and 40% (1,200 mg/kg) Moringa oleifera and 60% (900 mg/kg) Pleurotus ostreatus respectively. The rest of the diabetic rats were treated with 100%             (3,000 mg/kg) Moringa oleifera, 100% (1,500 mg/kg) Pleurotus ostreatus, while 7.1 mg/kg of metformin was used as the standard drug. The effect of the combined treatment of the extracts of Moringa oleifera and Pleurotus ostreatus on the diabetic animals were monitored by measuring the blood glucose, serum amylase and lipid profile (total cholesterol, triglyceride and HDL) in the blood samples. Results: The combined ethanol leaf extracts of Moringa oleifera and Pleurotus ostreatus significantly (p<0.05) lowered the concentration of blood glucose, amylase concentration and cholesterol in the diabetic rats been treated when compared with the diabetic control rats that were not treated. Conclusion: This study concluded that the combined ethanol extracts of Moringa oleifera and Pleurotus ostreatus produced a significant hypoglycemic effect in diabetic rats and are capable of controlling hyperlipidemia.