In vitro Multiplication of Oroxylum indicum (L.) through Apical and Axillary Buds and Determination of Genetic Fidelity Using RAPD Markers

Abstract

Present study involves to develop a protocol for large scale multiplication of Oroxylum indicum L. plants through apical and axillary buds using in vitro techniques. The apical and axillary buds were cultured on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzylaminopurine (BAP) and kinetin. The maximum number of shoots were observed on MS medium supplemented with 8.88 µM of BAP. Rooting was observed in half-strength MS medium supplemented with 8.88 µM of BAP and 7.38 µM of Indole-3-butyric acid. The well-developed plants were hardened in peat and perlite (1:1) and acclimatized in green house and 98% survival rate was observed and then shifted to field for cultivation. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic fidelity of the micropropagated plants. Ten different decamer primers were used to amplify genomic DNA from in vitro field grown plants and the mother plant to assess the genetic stability. The RAPD profile analysis revealed that micropropagated plants has no somaclonal variations with the mother plants. These results suggest that the micropropagation protocol developed by this study for rapid in vitro multiplication is appropriate and applicable for clonal mass propagation of Oroxylum indicum which helps in silviculture developments.