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Research Article Open access CC BY 4.0

Utilization of Nanobodies in Immunoturbidimetric Assays

Yue Lei Peng, Hao Jie Han, Bing Bing Du, Run Ying Zeng, Xin Yang

Journal of Advances in Biology & Biotechnology · pp. 44–52 · Published 7 Sep 2023

10.9734/jabb/2023/v26i7646

Abstract

Aims: Nanobodies, characterized by their small size, accessibility, and high yield, are emerging as advantageous tools in various applications.  Despite these benefits, their integration into the formulation of immunoturbidimetric reagents has remained limited since their discovery. Methodology: This study utilized nanobodies specific to cystatin C to formulate immunoturbidimetric reagents and compared them with traditional rabbit polyclonal antibody reagents.  Results: The findings revealed that the multiantibody affinity of Cys-rPAB surpasses that of Cys-VHH-104 and Cys-VHH-704 nanobodies, although the difference is not statistically significant. Upon evaluating the reagent formulation performance, both types of antibodies were found to satisfy the prerequisites of conventional immunoturbidimetric assay reagents.  The correlation coefficient R2 in the statistical analysis of the clinical results was 0.9986, signifying a high degree of consistency. Conclusion: These findings underscore the feasibility and considerable economic potential of employing nanobodies in immunoturbidimetric reagents.

Nanobodies immunoassay affinity cystatin C

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