Utilization of Agro-Industrial Waste Residues for the Production of Amylase from Aspergillus oryzae IIB-6
B. Fatima, Z. Hussain, M. A. Khan
Biotechnology Journal International · pp. 350–365 · Published 26 Feb 2014
10.9734/BBJ/2014/7915Abstract
Aims: The aim of this study was to evaluate different solid substrates and moistening agents for the production of an extracellular 1, 4-α-D-glucan glucohydrolase from a newly isolated Aspergillus oryzae IIB-6 by solid state fermentation and optimization of culture conditions for the maximal production of enzyme. Study Design: Six different agro-industrial waste residues (rice straw, rice bran, corn flakes, wheat bran, wheat flakes, and grinded wheat kernel) were procured from the local market. These substrates (10 g) were moistened (1:1) with different moistening agents (distilled water, tap water, mineral salts solution (FeSO4.7H2O 0.02, MgSO4.7H2O 1.0, (NH4)2SO4 4.0, KH2PO4 0.6, K2HPO4 1.4 mg/gds at ph 5), 0.1 N HCl, sodium acetate buffer (pH 5.5), sodium phosphate buffer pH 7.5) and screened for the production of 1,4-α-D-glucan glucohydrolase for 96 hours in static cultures. The substrate and moistening agent that gave maximum enzyme production were selected and their fermentation conditions were further optimized. Place and Duration of Study: Institute of Industrial Biotechnology (IIB), GC University Lahore, Kachery Road Lahore, Pakistan, between February 2011 and March 2012. Methodology: The levels of selected solid substrate, moistening agent and fermentation conditions such as pH, temperature, time of incubation, inoculum size etc. were optimized by one variable at a time method. Results: Wheat bran at the level of 5 g and mineral salts solution containing FeSO4.7H2O 0.016, MgSO4.7H2O 0.8, (NH4)2SO4 3.5, KH2PO4 0.48, K2HPO4 1.12 mg/gds gave relatively best enzyme production. The maximum enzyme activity 7800 U/gds (407 Umg-1 protein) were achieved when wheat bran with 80% moisture content was incubated at 30°C, pH 5, after inoculating with 10% spore suspension (1.2×106 CFU/gds) for 72 h. Conclusion: Aspergillus oryzae IIB-6 was a good producer of 1,4-α-D-glucan glucohydrolase in wheat bran medium containing mineral salts as an additional trace elements so that it can be used for biotechnological purposes.
References (25)
- 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
- 2 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
- 3 Straw and other fibrous by-products as feed
- 4 Coconut oil cake––a potential raw material for the production of α-amylase [DOI]
- 5 Amylase production in solid state fermentation by the thermophilic fungus Thermomyces lanuginosus [DOI]
- 6 Tannase production by Paecilomyces variotii [DOI]
- 7 Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species [DOI]
- 8 Chemical composition of kernel structures.
- 9 Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate [DOI]
- 10 Optimisation studies on neomycin production by a mutant strain of Streptomyces marinensis in solid state fermentation [DOI]
- 11 Effect of Fermentation and Particle Size of Wheat Bran on the Antinutritional Factors and Bread Quality [DOI]
- 12 Enhanced lignin peroxidase synthesis by Phanerochaete Chrysosporium in solid state bioprocessing of a lignocellulosic substrate [DOI]
- 13 Solid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues [DOI]
- 14 Production of starch-gel digesting amyloglucosidase by Aspergillus oryzae HS-3 in solid state fermentation [DOI]
- 15 Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture [DOI]
- 16 Utilization of sugarcane bagasse for solid-state fermentation and characterization of α-amylase from Aspergillus flavus isolated from Muthupettai Mangrove, Tamil Nadu, India.
- 17 Effects of Environment on Bacterial Wall Content and Composition [DOI]
- 18 Biosynthesis of glucoamylase from Aspergillus niger by solid-state fermentation using tea waste as the basis of a solid substrate [DOI]
- 19 Optimization of glucoamylase production by Colletotrichum sp. KCP1 using statistical methodology [DOI]
- 20 Influence of rice bran, oat bran, and wheat bran on cholesterol and triglycerides in hamsters
- 21 Efficient and simple extraction of mouldy bran in a pulsed column extractor for recovery of amyloglucosidase in concentrated form [DOI]
- 22 Purification and characterization of glucoamylase of Aspergillus terreus NA‐170 mutant [DOI]
- 23 Glucoamylase from a newly isolated Aspergillus niger FME: Detergent-Mediated production, purification, and characterization [DOI]
- 24 Production of Glucose by Starch and Cellulose Acid Hydrolysis and its Use as a Fuel in Low-Temperature Direct-Mode Fuel Cells [DOI]
- 25 Kinetics of improved 1,4-alpha-D-glucan glucohydrolase biosynthesis from a newly isolated Aspergillus oryzae IIB-6 and parameter significance analysis by 2-factorial design [DOI]
Cited by 9
Bilqees Fatima, Muhammad Mohsin Javed · Biomass Conversion and Biorefinery · 2020
Charu Gupta · Agri-food Waste Valorisation · 2023
Nissreen Abu-Ghannam, Elena Balboa · Sustainable Recovery and Reutilization of Cereal Processing By-Products · 2018
D. E. Mohamed, A. M. Alian, R. M. Mohamed · Food systems · 2024
Showing 4 of 9 known citations — external sources report more than can currently be individually listed.
Related research
- Production of Glucoamylase, α-amylase and Cellulase by Aspergillus oryzae F-923 Cultivated on Wheat Bran under Solid State Fermentation — shares topic coverage
- Impact of Solid-State Fermentation on Nutrient Profile, in vitro Gas Production Dynamics, and Methane/Carbon Dioxide Emissions of Selected Tropical Forages — shares topic coverage
- Comparative Analysis of the Chemical Indices of Oils Extracted from Local and Imported Banana Peels in Senegal — shares topic coverage
- Utilization of Byproducts in the Diet of Oreochromis niloticus: Impact of Probiotic Supplementation on Zootechnical Performance and the Biochemical Composition of the Flesh — shares topic coverage
- Survey on the use of Peanut Haulms (Arachis hypogaea L.) in Livestock Feeding in the Urban Area of Korhogo (Côte d’Ivoire) — shares topic coverage
Article metrics
Real usage data collected on this platform.
5
Page views
0
PDF downloads
0
Outbound clicks
9
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.