Skip to content
Research Article Open access CC BY 4.0

Using of Somatic Embryogenesis for Improving Bolting-resistant New Local Cultivars of Heirloom Carrot Cultivars (Daucus carota) Under Egyptian Conditions

H. H. Hamed, M. Khalil Mohamed

Asian Journal of Agricultural and Horticultural Research · pp. 161–177 · Published 23 Jun 2023

10.9734/ajahr/2023/v10i4257

Abstract

Foreign carrot varieties with desirable morphological traits for the local and global markets must bloom in low temperatures for a long period of time in Egypt, but these climatic conditions are not available for flowering and seed production for these varieties, so it is common to import carrot seeds from abroad. On the other hand, Local cultivars bloom earlier when planted later than the advised date. Due to the heat, it cannot be grown throughout the summer. Pushing the somatic cells to divide and create a new polarity is necessary for the induction of tissues in the lab and the creation of somatic embryos. It was shown that several auxins play a crucial role in the development of these embryos, particularly 2, 4-D (2,4-dichlorophenoxyacetic acid). This experiment was carried out to investigate the effect of five procedures in a tissue culture laboratory using two concentrations of 2, 4-D at a concentration of 2.5 and 5 mg/l for indirect somatic embryos induction from sexual embryos produced from culturing carrot  (Daucus carota) seeds of Carrot cv. Kuroda (an heirloom variety). The results confirmed by scanning electron microscopy showed that it is crucial to compare the various processes used to create somatic Embryogenesis to assess their effectiveness. The four stages of somatic embryos, globular, heart, torpedo, and cotyledon form, were observed on the formed callus. The highest amounts of callus fresh weight, the number of globular-shaped stage embryos, the number of heart-shaped stage embryos, the number of torpedo-shaped stage embryos, the number of cotyledon-shaped stage embryos, and the number of seedlings had been recorded in the media supplemented with 2, 4-D at a concentration of 5 mg. This study was able to produce somatic embryos of the foreign carrot cv. Kuroda, Plants obtained from somatic embryos are included in selection programs to produce varieties resistant to early flowering under Egyptian conditions. These results constitute a step in the way in the field of improving bolting-resistant new local cultivars of heirloom carrot cultivars (Daucus  carota) under Egyptian conditions.  

Carrot Daucus carota L somatic embryogenesis seed production 2,4-D

References (28)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Statistical Procedures for Agricultural Research. [DOI]
  3. 3 Statistical Methods -6th ed.-
  4. 4 Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana) [DOI]
  5. 5 Regulation of Somatic Embryogenesis in Higher Plants [DOI]
  6. 6 A transcriptional view on somatic embryogenesis [DOI]
  7. 7 Induction of somatic embryogenesis as an example of stress-related plant reactions [DOI]
  8. 8 Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. — an important medicinal plant [DOI]
  9. 9 Artificial seeds and their applications [DOI]
  10. 10 Azacitidine (5-AzaC)-treatment and mutations in DNA methylase genes affect embryogenic response and expression of the genes that are involved in somatic embryogenesis in Arabidopsis [DOI]
  11. 11 The Role of the Auxins During Somatic Embryogenesis [DOI]
  12. 12 New insights into plant somatic embryogenesis: an epigenetic view [DOI]
  13. 13 Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb. [DOI]
  14. 14 Encapsulation of Somatic Embryos in Synthetic Seed Coats [DOI]
  15. 15 Propagation of Valeriana wallichii DC. using encapsulated apical and axial shoot buds [DOI]
  16. 16 The role of auxins in somatic embryogenesis of Abies alba [DOI]
  17. 17 Localization and transport of indole-3-acetic acid during somatic embryogenesis in Coffea canephora [DOI]
  18. 18 Somatic Embryogenesis. An Overview [DOI]
  19. 19 Somatic Versus Zygotic Embryogenesis: Learning from Seeds [DOI]
  20. 20 Genome-wide analysis of transcription factors during somatic embryogenesis in banana (Musa spp.) cv. Grand Naine [DOI]
  21. 21 Biological techniques in electron microscopy [DOI]
  22. 22 Differential gene expression in embryogenic and non-embryogenic clusters from cell suspension cultures ofCoffea arabica [DOI]
  23. 23 Encapsulation of cauliflower (Brassica oleracea var botrytis) microshoots as artificial seeds and their conversion and growth in commercial substrates [DOI]
  24. 24 Insights into the conversion potential of Theobroma cacao L. somatic embryos using quantitative proteomic analysis [DOI]
  25. 25 Plant Regeneration from Somatic Embryogenic Suspension Cultures of Date Palm [DOI]
  26. 26 Embryogenesis induction of carrot (Daucus carota L.) in isolated microspore culture [DOI]
  27. 27 Interplay Between Auxin and Cytokinin and Its Impact on Mitogen Activated Protein Kinase (MAPK) [DOI]
  28. 28 Static suspension culture of carrot somatic embryos [DOI]

Cited by 4

Evaluating the Performance of the Carrot Slicer Machine

Amanuel Erchafo · Black Sea Journal of Agriculture · 2025

An efficient and easy-to-use protocol for induction of haploids in cucumber through parthenogenic embryo development

Pradeepkumara N, Chander Parkash, Reeta Bhatia · Botanical Studies · 2024

Showing 2 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

25

Page views

0

PDF downloads

5

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.