Use of Palm Oil Mill Effluent as Medium for Cultivation of Chlorella sorokiniana
Charles Ogugua Nwuche, Doris Chidimma Ekpo, Chijioke Nwoye Eze, Hideki Aoyagi, James Chukwuma Ogbonna
Biotechnology Journal International · pp. 305–316 · Published 19 Feb 2014
10.9734/BBJ/2014/6921Abstract
Aims: Palm oil mill effluent (POME) erodes the principal biophysical characteristic of both soil and water when discharged untreated but could be exploited as medium for microalgae cultivation due to its vast mineral contents. Place and Duration of study: POME samples were collected from a local palm oil processing mill at Nsukka, Enugu State, Nigeria. A part of the study was done at the Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan while the rest at the University of Nigeria, Nsukka between March and September, 2012. Methodology: Chlorella sorokiniana C212 was grown in several Batches (A-D) of POME supplemented with urea (60 mg/L) before subjecting to different sterilization protocols. Cultivation was conducted in shaker flasks at 150 rpm, 1 vvm, 3000 lux and pH 7.0±0.2. Results: The filter sterilized Batch (B) promoted the highest (1070±30 mg/L) dry cell weight (DCW), lipid (156±12 mg/g-cell) and chlorophyll (1.59±0.11 mg/g-cell) contents while chemical oxygen demand (COD) decreased by 45±08%. The autoclaved medium (Batch A) gave the least DCW (310±20 mg/L), lipid production (40±05 mg/g-cell) and chlorophyll content (0.58±0.02 mg/g-cell) while COD reduced by 20±04%. The highest COD decrease (70±05%) was achieved in the unsterilized Batch (D). Batch B was most positively affected by dilution because at 75% concentration, DCW increased to 1360±30 mg/L, lipid contents to 174±10 mg/g-cell, chlorophyll to 1.87±0.14 mg/g-cell the while COD declined by 63±03%. Conclusions: POME has potential for use in microalgae cultivation with significant saving in treatment costs.
References (23)
- 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
- 2 Colorimetric Method for Determination of Sugars and Related Substances [DOI]
- 3 A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION [DOI]
- 4 Biodiesel from microalgae [DOI]
- 5 Commercial applications of microalgae [DOI]
- 6 Purification and properties of unicellular blue-green algae (order Chroococcales) [DOI]
- 7 Valuable products from biotechnology of microalgae [DOI]
- 8 Factors limiting bioremediation technologies [DOI]
- 9 PHOTOACCLIMATION OF PHOTOSYNTHESIS IRRADIANCE RESPONSE CURVES AND PHOTOSYNTHETIC PIGMENTS IN MICROALGAE AND CYANOBACTERIA 1 [DOI]
- 10 Bioactive Compounds from Cyanobacteria and Microalgae: An Overview [DOI]
- 11 Water recycling from palm oil mill effluent (POME) using membrane technology [DOI]
- 12 The discovery and development of marine compounds with pharmaceutical potential [DOI]
- 13 Isolation, spectroscopy and selective phytotoxic effects of polyphenols from vegetable waste waters [DOI]
- 14 Olive oil mill effluent (OOME). New substrate for biosurfactant production [DOI]
- 15 Nutritional values of chironomid larvae grown in palm oil mill effluent and algal culture [DOI]
- 16 Sequential heterotrophic/autotrophic cultivation – An efficient method of producing Chlorella biomass for health food and animal feed [DOI]
- 17 Effect of water deficit on microbial characteristics in soil amended with sewage sludge or inorganic fertilizer under laboratory conditions [DOI]
- 18 A novel approach for medium formulation for growth of a microalga using motile intensity [DOI]
- 19 Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis [DOI]
- 20 Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils [DOI]
- 21 Cultivation of Chlorella sp. as Biofuel Sources in Palm Oil Mill Effluent (POME) [DOI]
- 22 Potential of Palm Oil Mill Effluent (POME) as Medium Growth of Chlorella sp for Bioenergy Production
- 23 Basic Culturing Techniques [DOI]
Cited by 87
Suchitra Gaur, Mehak Kaur, Rishu Kalra · Heliyon · 2024
K M Palanisamy, P Paramasivam, S Jayakumar · IOP Conference Series: Earth and Environmental Science · 2021
Nita Ariestiana Putri, Resti Nurmala Dewi, Rizka Lestari · Jurnal Rekayasa Kimia & Lingkungan · 2023
Sanjay Kumar Gupta, Faiz Ahmad Ansari, Amritanshu Shriwastav · Journal of Cleaner Production · 2016
Shih-Hsin Ho, Yi-Di Chen, Wen-Ying Qu · Biofuels from Algae · 2019
Nugroho Adi SASONGKO, Ryozo NOGUCHI, Tofael AHAMED · Journal of the Japan Institute of Energy · 2015
Patrícia Verdugo Pascoal, Dágon Manoel Ribeiro, Carolina Ribeiro Cereijo · PLOS ONE · 2021
Farah Minaoui, Zineb Hakkoum, Amer Chabili · Algal Research · 2024
Hanaa H. Morsi, Mostafa M. El-Sheekh, Hamed Eladel · Water · 2023
Wai Yan Cheah, Pau Loke Show, Joon Ching Juan · Energy Conversion and Management · 2018
Showing 20 of 87 known citations — external sources report more than can currently be individually listed.
Related research
- Assessment of Knowledge and Attitude Regarding Sterilization — shares topic coverage
- Barbing Equipments: Tools for Transmission of Tinea Capitis — shares topic coverage
- In vitro Sterilization of Alstroemeria hybrida cv. Pluto — shares topic coverage
- Factory Production Process of Shiitake Mushrooms — shares topic coverage
- A Study on Cold Plasma for Food Preservation — shares topic coverage
Article metrics
Real usage data collected on this platform.
91
Page views
0
PDF downloads
25
Outbound clicks
87
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.