HPLC Determination of the Eight Constitutes in Portulaca oleracea L. from Different Locations
Junjun Ai, Aijing Leng, Xiaomei Gao, Wenjie Zhang, Di Li, Liang Xu, Xixiang Ying
European Journal of Medicinal Plants · pp. 156–164 · Published 10 Oct 2014
10.9734/EJMP/2015/13253Abstract
Aims: The objective of the present research was to simultaneously determine the eight constituents in Portulaca oleracea L. from different locations by a sensitive and specific high-performance liquid chromatographic (HPLC) method, including caffeic acid (CA), p-coumaric acid (p-CA), scopoletin (SCOP), ferulic acid (FA), quercetin-3-O-rhamnoside (QR), quercetin (QUER), apigenin (APIG) and bergapten (BERG) using rhein as the internal standard. Methodology: With gradient elution procedure and mobile phase A (0.1% formic acid) and B (acetonitrile) ratio linear changing, the wavelength was set to 320 nm to determinate the eight constituents. Result: The method validation presented good accuracy with recoveries in the range of 93.67-113.3% and good precision with RSD values less than 12.1%.The results indicated that there existed the significant differences of the eight constituents in P. oleracea L. from 20 different locations. Conclusion: The results can be used to reasonably assess the drug quality and exploit P. oleracea L.
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