Comparative Study on Phytochemical Extraction, Antibacterial and Synergistic Activity of Zingiber rubens with Tetracyclin on Homo sapiens and Gallus domesticus Originated Enteropathogenic Bacteria

Abstract

Introduction: Nature is a rich source of medicinal plants, these medicinal plants used as a therapeutic agent from thousands years. At present medicinal properties of many plants compiled and these plants are source of many drugs, but properties of some medicinal plants is still unknown. The main object of this study is determination of phytochemical of Zingiber rubens, and biological activity of these active compounds against enteropathogenic microbes for determination of antibacterial activity and synergetic activity between plant extract and Tetracycline. Methods: Firstly tubers of Zingiber rubensare shade dried and then extraction completed from powder form of plant material with different kinds of solvents (water, ethanol, petroleum ether and chloroform). After extraction their phytochemical constituents resolute through biochemical analysis and their antibacterial and synergetic activity examined through disc diffusion method on different strains of human and cock isolated enteropathogenic bacterial species. Results: In our study we observed that organic solvent extract comprise more active compounds than other extract and the antibacterial activity of these organic extract is also higher than aqueous extract. In aqueous extraction between the temperatures at 30-50°C maximum numbers of phytochemicals are screened. Chloroform extract shows maximum inhibitory activity along and with Tetracycline against E. coli (12 mm/25 mm), cold water extract show minimum inhibitory activity along and with Tetracycline against S. aureus (6 mm/13.5 mm). Antibacterial activity expressed in terms of zone of inhibition. Conclusion: from the results we concluded that extraction depends on nature of solvent and extraction temperature. Temperature between 30-50°C appropriate for organic and aqueous extraction.  At this temperature maximum number of photochemical screened in warm water and all organic solvent extract. Zingiber rubens contained both antibacterial and synergetic activities. Antibacterial activity of Tetracycline increases, due to the synergetic activity between Tetracyclin and Zingiber rubens phytochemicals.