Comprehensive Physicochemical Characterization and GC-MS Profiling of Neelibhringadi Kera Tailam: A Traditional Ayurvedic Oil Formulation for Hair Growth

Abstract

Introduction: Neelibhringadi Kera Tailam is a traditional Ayurvedic herbal oil formulated for promoting hair health, addressing issues such as hair loss, premature greying, scalp irritation, and infections. This study evaluates its composition, preparation, organoleptic, physicochemical, phytochemical, and GC-MS profiles to substantiate its therapeutic efficacy. Materials and Methods: The raw herbal materials were cleaned, processed into juices and a homogeneous paste (kalka).Coconut oil was mixed with the kalka and juices in a vessel, stirred and heated to mridu paka, followed by addition of the milks and continued heating until kharapaka; the mixture was then filtered through cotton cloth into a vessel with powdered Anjana, stirred, and stored in a clean container.Analyses were conducted per Ayurvedic Pharmacopoeia of India (API) standards. Results: Organoleptic evaluation revealed a bluish green color, characteristic herbal odour, and viscous oily consistency. Physicochemical parameters met API standards, while phytochemical screening confirmed the presence of key bioactive compounds like steroids, alkaloids phenols & Saponins. GC-MS identified 25 compounds of which 18 major compounds were selected for their potent properties supporting hair growth, antimicrobial action, and scalp nourishment. Conclusion: The analytical data support Neelibhringadi Kera Tailam promotes hair health by penetrating the hair shaft, stimulating growth and restoration, and exerting antimicrobial, antioxidant, anti-inflammatory, and hair loss–preventive effects.