Dew Formation and Anaerobiosis in Anaerobic Jars

Abstract

Introduction: This study aimed to prove whether the palladium catalyst affects the dew formation time in the anaerobic cover and whether anaerobiosis can be achieved without a palladium catalyst. Materials and Methods: This research is a laboratory experimental study with a non-randomized control group design. In this study, replication was carried out. The amount of replication used is 10. The palladium catalyst was used in 10 experiments, whereas it was not used in ten other experiments. Results: The growths of Pseudomonas aeruginosa, Clostridium tetani, and Bacteroides fragilis were observed after 48-hour incubation. The time of appearance of water condensate was observed until 24-hour incubation. The time of appearance of water condensate in the palladium-contained anaerobic jar varied between 1.37 minutes and 3.33 minutes. On the other hand, water condensate did not appear in the without-palladium anaerobic jar. No growth of Pseudomonas aeruginosa was observed in the palladium-contained anaerobic jar; on the contrary, there were ten growths in the anaerobic jar without palladium. Eight growths of Clostridium tetani were observed in the palladium-contained anaerobic jar, whereas there was no growth without palladium. No growth of Bacteroides fragilis was observed in the without-palladium anaerobic jar, whereas seven growths were observed in the anaerobic jar containing palladium. The time of appearance of water condensate in the palladium-contained anaerobic jar was different from that in the anaerobic jar without palladium. Significant differences (p < 0,01) in anaerobiosis creation between the palladium-contained anaerobic jar and the without-palladium anaerobic jar were also clearly observed.