Performance of Brioler Chickens and mRNA Expression of Liver Fatty Acid Binding Protein Gene Fed with Soybean

Abstract

Aims: An experiment was conducted to investigate the effects of different levels of processed full-fat soy bean on performance, Lipid blood carcass quality and mRNA expression levels of liver fatty-acid binding protein (LFABP) in broiler chicks. Study Design: A total of 240 day-old male broilers (Ross 308) was used in a completely randomized design. Place and Duration of Study: Islamic Azad University, Kashmar Branch Farm, 42 days. Methodology: Experimental treatments included a control treatment (corn-soybean meal), levels 8 and 12% roasted full-fat soybean and levels 8 and 12% extruded full-fat soybean in rations .Each dietary treatment was assigned to four replicate pens holding 12 male broiler chicks. Roasting was conducted for 20 seconds at a temperature of 110°c and extruding for 90 seconds at a temperature of 170°C. Weight gains, feed intake and feed conversion ratio were measured at the end of each experimental period (10, 24, and 42 days of age). At the end of experimental periods, blood samples were collected from wing vein to measure the serum trigeliserids cholesterol, LDL and HDL levels. Results: The effect of diets on feed conversion ratio was significant at each periods, as well as total period although it was just significant on increasing of growth rate and decreasing of feed intake at grower and finisher periods (p<0.05). Results of the carcass evaluation were not significant. Reduction of pancreas relative weight was only observed in treatments contained roasted and extruded soybean. Replacing extruded and roasted soybean with soybean meal caused a reduction trend of blood cholesterols, triglyceride and LDL, which was statistically significant. The results showed that the mRNA expression of LFABP in treatments containing 12% roasted full-fat soybean and extruded full-fat soybean was higher than the control (p<0.01). Conclusion: The results showed improvement of performance by including roasted or extruded soybean in broiler diets. The mRNA level of LFABP would be increased by increasing the full-fat soybean levels.