Assessment of Enzymic, Non-enzymic Antioxidants and In vitro Free Radical Scavenging Activities of Different Extracts of Leaves and Roots of Eclipta alba

Abstract

To obtain the scientific evident for antioxidant properties of Eclipta alba by using enzymic, non-enzymic antioxidant activity and in vitro free radical scavenging activity. This study was conducted on different organic solvents and hot aqueous extracts of leaves and root of Eclipta alba were tested for enzymic, non-enzymic antioxidant activity and in vitro free radical scavenging activity. Among the enzymic antioxidants tested interestingly, in leaves and roots, glutathione reductase showed potent activity of 178.2±4.5 U/mg, 120±9.0 U/mg respectively. Carotenoids expressed 305.5±1.70 mg/g being the most effective among the other non- enzymic antioxidants tested. All the extracts of leaves and roots exhibited a potent inhibition against DPPH free radical, hydrogen peroxide, lipid peroxidation, nitric oxide and superoxide generation. The data obtained in the in vitro tests clearly established the antioxidant potency of all extracts. Hence, overall results suggested that E. alba is a promising source of bioactive compounds that can be exploited as antioxidants in food products as well as in pharmaceutical therapeutic and cosmetic industry use.