Hypobaric Pressure Effects on Gene Expression, as a Physiological Response of Canola Varieties

Abstract

Aims: The main purpose of our study was to determine the expression levels of genes, which respond to low pressure in canola (Brassica napus L.) varieties Californium, Orkan, Jura and Elvis. Study Design: Canola (Brassica napus L.) varieties (Californium, Jura, Orkan and Elvis) placed within specially designed low pressure cabinets were exposed to low pressure (40 Torr ≅ 53 kPa) for durations of 1, 2 or 3 days. Total RNA was isolated from the plants analyzed for genes, OsNCED, OsABA8, OsZEP and TMAC2 by RT-PCR technique. For control, housekeeping gene β-actin was used. As a result OsABA8ox 1, 2, 3 and OsNCED showed increase in expression levels. Methodology: Canola (Brassica napus L.) varieties Californium, Orkan, Jura and Elvis were provided by the Black Sea Agricultural Research Institute, Samsun – Turkey. Seeds were sown and placed in the plant growth chamber. The 14 days old plants were exposed to low pressure in the low pressure cabinet. RT-PCR reactions were performed in one-tube reaction according to manufacturer’s protocol (Access Quick RT-PCR System, Promega, A1701). Results: The PCR products of OsNCED, OsZEP, OsABA, TMAC2 separated by 2% agarose gel electrophoresis were found to be approximately between 200-300bp. The PCR products of OsABA8 gene (~750bp) in Jura was determined to be increased compared to that of control group. Change of expression of OsABA8 gene (~750bp) in varieties of Jura and Orkan were determined and compared to that of control group. OsABA8 gene region (~300bp) in varieties of both Californium and Elvis, whose expression of OsNCED gene region only (~300bp) in varities of Jura and Orkan were determined and compared to that of control group. Conclusion: Any changes have been determined in the expression of TMAC2 gene which supports the other studies in the literature. As a consequence of this, results obtained from our study have the feature that can give a new direction to other studies. In addition to this, because of there is no yet such a study related to low atmospheric conditions, this study has the characteristics of being the first and fundamental study with this speciality.