In vitro Anticoagulant Effect of Aqueous Garlic (Allium sativum) Extract on Human Plasma Coagulation

Abstract

Background: Bleeding disorders is a dangerous situation affect population, and their treatment may have side effects, very expensive and not available so people resorted to use the herbal medicine because it’s safe, available and with low cost, One of them is Garlic (Allium sativum), in which there is a previous study confirming their activity as anticoagulant. Objective: The present study aimed to detect the In vitro anticoagulant effect of Garlic extraction (Allium sativum) on normal human plasma. Materials and Methods: This was experimental study, 15 normal blood samples were collected and treated with varying concentrations (100%, 50%, and 25%) of aqueous extracts of Garlic (Allium sativum). The PT and APTT were measured for each treatment group and control group. Statistical analysis was performed to determine the significance of the results by SPSS version 25. Results: The median of prothrombin time in three different concentrations of extraction (Allium sativum) was measured in fifteen (15) samples which was prolonged than the median of control group. The highest median of prothrombin time was founded when plasma incubated with 100% aqueous extraction (39 sec) compared to the median of control (12sec) with the P -value is less than 0 .05. The median of activated thrompoblastin time (APTT) in three different concentrations of extraction (Allium sativum) in fifteen (15) samples which was prolonged than control group and the highest median of APTT time was founded when plasma incubated with 100% aqueous extraction (68 sec) compared to the median of control (27 sec) with P- value less than 0.05. Conclusion: Through our investigation of prothrombin time (PT) and activated Partial thromboplastin time (APTT) using aqueous Garlic (Allium sativum) extract, we observed significant prolongation of both PT and APTT with increase garlic concentration that confirm the anticoagulant activity of garlic extract.