Biopotential of Some Trichoderma spp. against Growth of Fusarium oxysporum f. sp. Vasinfectum Causal Agent of Cotton Wilt

Abstract

Evaluation of the antagonistic activity of some Trichoderma species by direct confrontation method was used in this study. The mycoparasitism inhibitory effects of Trichoderma species on the growth of the causal agent of cotton Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) were investigated by dual culture under in vitro condition. Seventeen isolates of Trichoderma and one strain of Fusarium oxysporumf. sp. vasinfectum (FOV) were used. These different isolates of Trichoderma were in vitro confronted to FOV. The invasion ability of antagonist colonies was measured during 14 days. Thus, at the end of these control trials, isolates of Trichoderma have controlled the growth of FOV. Indeed, FOV is confined to a small area and is unable to extend its growth after the third day of incubation in the culture room at 30°C. Beyond this period and after then days, they never sporulated, revealing the inhibitory action of Trichoderma against FOV. Consequently, the Trichoderma isolates were found able to biologically control of FOV growth.