Single Cell Protein Production from Torula Yeast (Cyberlindnera sp.) Using Banana Peel Hydrolysate

Abstract

Introduction: Single cell protein is a source of protein derived from one-celled organisms (algae, yeasts, fungi or bacteria) grown on different carbon sources and is used as a substitute for protein-rich supplement for human and animal feeds. Methods: In this study, ten yeast isolates from saw dust samples that were obtained from small scale wood processing enterprises from Gondar town, Ethiopia were screened and identified through morphological, cultural and physiological tests. Results: From the different yeast isolates, an isolate SDY6 was found to be Torula yeast (Cyberlindnera sp.). The biomass production capacity of this yeast isolate was evaluated using banana peel hydrolysate under batch and aerobic conditions in which pH and temperature were adjusted to 4.5 and 30°C, respectively. (NH4)2SO4 was added to the banana peel hydrolysate to increase biomass yield. The produced biomass was measured at 0, 24, 48, 72 and 96 h. Maximum biomass of 8.82±1.21 g/L was produced at 48 h incubation. On the other hand, crude protein content of the product was analysed using Kjeldahl apparatus. About 10.25±2.73% of maximum crude protein content was obtained by this yeast. Conclusion: In conclusion, a higher biomass yield and crude protein content can be obtained from Torula yeast (Cyberlindnera sp.) using banana peel hydrolysate as a substrate.