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Research Article Open access CC BY 3.0

The Possibility of Using Fibers as a Prebiotic in Making of Probiotic Based On-Some Dairy Products

Gehan H. Bisar, Kh. El-Saadany, A. Khattab, Wedad M. El Kholy

Microbiology Research Journal International · pp. 678–697 · Published 12 Mar 2014

10.9734/BMRJ/2014/8587

Abstract

Probiotic microorganisms were found to affect the host health beneficially when found in an a certain count not less than 106 CFU/g (Colony-forming unit / gram), and they have some benefits as protection from cancer, relief of lactose intolerance, reduce the risk from diarrhea, normalize the bowel movement, and enhance the immune functions, reduce cholesterol level and reduce the risk of eczema. This study was carried out to examine some of fibers and polysaccharide for their assimilation by some lactic acid bacterial strain specially known for their probiotic effect. It was concluded from the present study the following: studying the capability of Esherichia coli (E. coli), bifidobacteria and 9 strains related to lactic acid bacteria included in assimilating 7 different (polysaccharides, fibers and other materials) included (Polydextrose, Maltodextrine, inulin, prolia, resistant starch, wheat fiber and gumarabic) when substituted with dextrose inde Man, Rogosa and Sharpe (MRS) broth and incubated at their optimal temperature. The results revealed that the examined culture were varied in their assimilation of the 2% polysaccharides tested, furthermore maltodextrin, showed a good assimilation by Bifidobacterium longum ATCC 15707 (B. longum) and Lactobacillus acidophilus NRRLB1910 (L. acidophilus). The effect of certain concentrations (2, 3and 4%) of the selected (polysaccharides, fibers and other materials) on the growth activity of the lactic acid bacterial cultures tested in addition to E. coli (as a representative for coliform bacteria). The results revealed that upon increasing the concentration of the selected polysaccharides there was a remarkable decrease in pH compared to E. coli which showed contrast outcomes on which its pH were significantly higher than the tested bifidobacteria and lactic acid bacteria. Studying the effect of incubation duration and it’s relation on the selected (polysaccharides, fibers and other materials) assimilation by the tested lactic acid bacterial cultures. Results revealed that there is a direct proportion relation between long incubation timing and polysaccharide assimilation (indicated by decrease in pH). This decrease was very clear at 24 hours of incubation at the optimum temperature for each strain.  Upon studying the antagonistic effect between E. coli with B. longum ATCC 15707, L. acidophilus NRRLB 1910 and Lactobacillus reuteri B 14171 (L. reuteri) grown on modified MRS with 3% of each polysaccharide (polydextrose, maltodextrin and inulin). The change in the growth of these cultures combinations were determined by counting on MRS and Violet red bile agar (VRBA). It was shown that these (polysaccharides/fibers) challenged the growth of the probiotic bacteria and the count of E. coli (wild) E.W was lowered significantly due to the inhabitation effect of the used probiotic bacteria. It was concluded that good results was shown from using the three polysaccharides/fibers (maltodextrin, inulin andpolydextrose) that was elected to base the rest of work on.

Probiotic lactic acid bacterial fibers polysaccharide

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