Identification, Analysis and Comparison of Human Alpha Tubulin Gene

Abstract

Tubulins are composed of filaments or thread like structures called as microtubules which plays an important role in structural composition of cytoskeleton. Tubulin comprise of various types and subtypes. They belong to family of globular proteins.α-tubulin’s are considered as a multigene family in various species. Sequence analysis of human, mouse and rat α-tubulin has enabled an updated nomenclature to be studied. Analysis of orthologues helps to study evolutionary relationships and to identify the sequence homology among all genetic variants. Researchers can predict various structural elements and can overcome many gene mapping problems. Purpose: To study and compare the evolutionary relationships, homologs, orthologues and putative protein domains. Materials and Methods: Sequence analysis of human, mouse, and rat α-tubulin gene has enabled an updated nomenclature to be studied. Analysis of orthologues helps to study evolutionary relationships and to identify the sequence homology among all genetic variants. Results: Comparative study was performed by using number of different bioinformatics tools and software’s like BLAST, Ensemble and Interpro. The nucleotide sequence of 1353 bp was retrieved from Genbank NCBI. The sequence was rich with many coding regions. The mRNA transcript was analyzed with 3 different splice variants. Various protein domain were analyzed which belongs to superfamily of TUBA3C gene associated protein. Conclusion: The analysis of TUBA3C gene and protein domain helped to predict the 3D structure of related proteins. The homology and similarity studies can help to predict the evolutionary relationships.