Switching between Heat Shock Proteins and Cold Inducible Proteins under Temperature Fluctuation in Solanum tuberosum L. Cultivars in In Vivo Condition
Biotechnology Journal International · pp. 101–112 · Published 17 Sep 2011
10.9734/BBJ/2011/495Abstract
Cross-talking between heat shock proteins (HSPs) and cold inducible proteins (CIPs) subsequent to combinational mild heat (35°C) and cold (8°C) stress was investigated in vivo for four cultivars of Solanum tuberosum L. viz. Kufri Pukhraj (PO), Kufri Jyoti (GS), Kufri Ashoka (KF) and Kufri Chandramukhi (CM) in the order of their decreasing thermotolerance, to understand how this economic crop adapts to extreme temperature fluctuation. We showed a time-course differential genotypic expression pattern for HSPs at 35°C for 10h and CIPs at 8°C for 12h time-lapse. Remarkably, we noted the disappearance of a housekeeping protein (HKP) of about 19.8KD at 2h, 35°C in GS absent in CM, KF and PO; but strongly expressed as CIPs at 8°C for all the cultivars. Furthermore, heat-stress led to an outstanding transient induction of HSP95.9, HSP83.6, HSP78.7, HSP70.7, HSP66.0, HSP54.1, HSP48.6, HSP43, sHSP38.3, sHSP35.3, sHSP29, sHSP22.5, sHSP17.8 and sHSP9.5 in GS at 6h, while HKP58.7, HKP55.5 and HKP43.7 were stably overexpressed in CM, KF and PO. Temperature switching from 35°C to 8°C upregulated HKP43.4, HKP54.6, CIP14.1 and HKP19.9 for all the cultivars. The recovery process 24h subsequent to this archetype switching was governed by overexpression of small(s)HSPs of about 25.4KD-14.1KD, HKP58.7 and HKP43.5 for all cultivars. Results suggest cross-talk protection for this paradigm-shift in temperature is chiefly conferred by isoforms of constitutively expressed HKPs, CIP19.9 and CIP14.1 in S. tuberosum L. Explicitly, this differential proteome change within 22h signify HKPs may not participate in thermotolerance as HSPs, but participate in cold acclimation as CIPs, recovery as sHSPs and even switch-off during heat-stress in some cultivars as depicted in GS.
References (18)
- 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
- 2 Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance [DOI]
- 3 Abiotic stress, the field environment and stress combination [DOI]
- 4 A CCLIMATIVE R ESPONSE TO T EMPERATURE S TRESS IN H IGHER P LANTS : Approaches of Gene Engineering for Temperature Tolerance [DOI]
- 5 Cold stress and acclimation – what is important for metabolic adjustment? [DOI]
- 6 Small heat shock proteins and stress tolerance in plants [DOI]
- 7 Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways [DOI]
- 8 The effect of climate change on global potato production [DOI]
- 9 Effect of High Temperature on Plant Growth and Carbohydrate Metabolism in Potato [DOI]
- 10 The Effect of Water, Sugars, and Proteins on the Pattern of Ice Nucleation and Propagation in Acclimated and Nonacclimated Canola Leaves [DOI]
- 11 Genotypic differences in the heat-shock response and thermotolerance in four potato cultivars [DOI]
- 12 An overview on the small heat shock proteins [DOI]
- 13 Heat Shock Proteins and Heat Shock Response in Plants
- 14 Production of high temperature tolerant transgenic plants through manipulation of membrane lipids.
- 15 Heat Shock Protein Synthesis and Thermal Tolerance in Wheat [DOI]
- 16 Possible effects of climate change on potato crops in Estonia [DOI]
- 17 Alterations of gene expression in potato (Solanum commersonii) during cold acclimation [DOI]
- 18 Effect of year, location, and harvest on susceptibility of cultivars to internal heat necrosis in North Carolina [DOI]
Cited by 4
Bengyella Louis, S. D. Waikhom, R. Jose · Microbial Pathogenesis · 2015
Bengyella Louis, S. D. Waikhom, P. Roy · BMC Genomics · 2014
Lina Bernaola Alvarado · 2012
Showing 3 of 4 known citations — external sources report more than can currently be individually listed.
Related research
- The Effects of Genotype and Irrigation Regime on PSII Heat Tolerance in Cotton — shares topic coverage
- Screening of Thermotolerant Acetic Acid Bacteria Involved in Cocoa Fermentation in Six Major Cocoa Producing Regions in Côte d’Ivoire — shares topic coverage
- Selection and Identification of a Newly Isolated Thermotolerant and Amylolytic Saccharomyces cerevisiae Strain for Ethanol Production — shares topic coverage
- A Review on Production Efficiency and Seasonal Disease Management in Mulberry Sericulture in Telangana, India — shares topic coverage
- Screening of Rice (Oryza sativa L.) Genotypes for High Temperature Stress under Temperature Induction Response — shares topic coverage
Article metrics
Real usage data collected on this platform.
56
Page views
0
PDF downloads
16
Outbound clicks
4
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.