Studies on Association of Seed Borne Mycoflora in Different Varieties of Black Gram [Vigna mungo (L.) Hepper] by Incubation Methods

Abstract

The present study was conducted to detect and identify seed-borne pathogenic fungi in some different varieties of black gram and their effect on seed germination. Six varieties of black gram (Indira urd-1, Pratap-1, KU-96, TPU-4, TIU-22) and local variety collected from village for experiment. The Eleven seed associated mycoflora recorded were Aspergillus flavus Aspergillus niger, Fusarium spp., Alternaria spp., Rhizopus spp., Penicillium spp., Curvularia spp., Cladosporium spp., Colletotrichum spp., Chaetomium spp. and Trichoderma spp. were detected and identified from the seeds of  different varieties of black gram  by using the standard blotter method, agar plate method, 2,4-D blotter method, roll paper towel method and deep freeze method. In all these methods, poor germination percentage was recorded in local variety, may be due to the presence of seed associated mycoflora with maximum frequency, whereas KU-96 variety has most extreme germination percentage due to the minimum frequency of seed associated mycoflora as compared to other varieties of black gram taken in the examination.