Spodoptera frugiperda (fall armyworm) Population Analysis in Maize-Growing Areas of Southeastern and South-Central Côte d'Ivoire

Abstract

Aims: This study focuses on the analysis of fall armyworm (Spodoptera spp.) populations in three maize-producing areas of Côte d'Ivoire. Study Design:  During the laboratory test, the tubes were arranged in completely randomized blocks. Place and Duration of Study: Central Laboratory of Biotechnology (Centre National de Recherche Agronomique); Sampling, between June 2024 and July 2025 Methodology: The incidence and severity of infestation were assessed. In addition, a diversity analysis was performed using the specific primers JM77 and JM76, targeting the mitochondrial cytochrome oxidase (COI) gene region on more than 250 isolates. Results: The results show significant variability in the level of Spodoptera frugiperda infestation across the studied locations. The southern zone showed higher incidence and severity rates in the towns of Grand-Lahou and Alepé, with 97% and 91.5% infestation rates, and 88% and 80% severity rates, respectively. Molecular analysis did not reveal population diversity with the molecular markers used. Conclusion: This study shows the use of different molecular markers, for an effective control of the armyworm population in maize orchards.