Spectrophotometric Analysis of Variation of Fluoride Content in Dentifrices – A Quantitative Study

Abstract

Background: A dentifrice and a toothbrush are universal armamentariums for oral hygiene maintenance. The presence of fluoride in commercial dentifrices aids in dental caries prevention. The fluoride content and compound are mentioned on the packaging along with the time of manufacture and its shelf life. Aim: To evaluate the fluoride concentration of dentifrices available commercially over a  period of time from the date of manufacture. To compare any variations in the fluoride concentration over time to the content present at manufacture. Methodology: This is an In vitro comparative observational study. 8 Standard dentifrices easily available Over the Counter were selected, of which 3 were for adult use, 2 were herbal and 3 were for kids. They were subjected to fluoride analysis and the fluoride content was assessed using spectrophotometric analysis, by a single blinded examiner. The test sample was compared to a standard solution, in a calibration curve to determine the fluoride concentration. Results: On analysis it was seen that adult toothpaste samples had fluoride concentration  lower than 1000ppm as mentioned on the label. One herbal toothpaste had fluoride concentration of 1039.62ppm which was higher than that mentioned on the label. One kids toothpaste had  fluoride concentration of 905.25ppm which was higher than that mentioned on the label. Conclusion: The toothpastes included in the study had dissimilar fluoride concentrations after analysis than the concentration mentioned on the label. Thus, waranting better laws and standardization methods for toothpastes.