Optimization of Fibrinolytic Enzyme Production from Bacillus sp. IND6 in Solid State Fermentation
Mohammed A. Almalki, D. S. Deepa Dhas, Ponnuswamy Vijayaraghavan
Journal of Scientific Research and Reports · pp. 1–8 · Published 16 Oct 2017
10.9734/JSRR/2017/36748Abstract
Aims: This study aimed to screen and optimize Bacillus sp. IND6 for fibrinolytic enzymes production. The process parameters were optimized to enhance the production of fibrinolytic enzyme in solid state fermentation using wheat bran substrate. Methodology: In this study fibrinolytic enzyme production was carried out using Bacillus sp. IND6 in solid-state fermentation. Wheat bran was used as the substrate. The process parameters were optimized using traditional one-variable-at-a-time approach. Results: The significant increase in fibrinolytic enzyme production was occurred when this organism was grown up to 72 h (1310 ± 31.6 U/g), at pH 9.0 (1417.4 ± 73.2 U/g) with 90% moisture content (1441.2 ± 77.4 U/g). Among the carbon sources, starch significantly enhanced enzyme production (1713.8 ± 53.9 U/g). Conclusion: Bacillus sp. IND6 could be the good source of fibrinolytic enzymes for various biotechnological applications.
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Łukasz Wajda, Magdalena Januszek · Biotechnological Applications of Biomass · 2021
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