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Research Article Open access CC BY 4.0

Serum Creatinine Determination: Comparison between Colorimetric (Jaffé) and Enzymatic Methods in Renal Transplant Recipients

Houda Kouame, Mohammed Benamo-Seghir, Ayoub Samih, Asmaa Morjan, Nabiha Kamal

Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 9–14 · Published 19 Jun 2023

10.9734/ajbgmb/2023/v14i3314

Abstract

Background: Serum creatinine is the most requested parameter in current practice in the context of functional exploration of the kidney. Its contribution to medical care is particularly decisive for kidney transplant patients. The aim of the study is to compare the colorimetric and enzymatic techniques available in our laboratory for the determination of creatinine in samples from kidney transplant patients. Methods: Prospective study carried out at the Biochemistry laboratory of the University Hospital Center (CHU) Ibn Rochd in Casablanca Morocco, over a period of 8 months, carried out on a series of 45 samples from kidney transplant patients who simultaneously benefited from a creatinine assay by colorimetric and enzymatic techniques. The samples were taken on dry tubes and analyzed on the Alinity ® Abbott analyzer. The statistical study of correlation was made by the SPSS software with calculation of the p-value and the coefficient of Pearson (r). Results: During the study period 45 samples were collected. Seventy percent of the prescriptions came from the Nephrology Department. The mean serum creatinine value by enzymatic technique was 39.04 ± 11.7 mg/l, and by colorimetric technique 38.24 ± 12.4 mg/l. The creatinine level by the two techniques was pathological in 69% of cases and normal in 31%. The correlation between the serum creatinine assay by enzymatic Versus colorimetric technique is positive with r=0.994 and P-value<0.001. Conclusion: According to literature, colorimetric techniques are sensitive to interference with pseudochromogenic substances, while enzymatic techniques are standardized due to their specificity and performance but remain more expensive. In our study the correlation between the two techniques was positive with a correlation equation: (y = 0.82 + 1*x). According to our results, no significant difference between the two techniques was observed in our laboratory.

Enzymatic creatinin colorimetric creatinine renal transplant recipient

References (15)

  1. 1 Recommendations for Improving Serum Creatinine Measurement: A Report from the Laboratory Working Group of the National Kidney Disease Education Program [DOI]
  2. 2 Creatinine Measurement: State of the Art in Accuracy and Interlaboratory Harmonization [DOI]
  3. 3 Creatinine determination according to Jaffe--what does it stand for? [DOI]
  4. 4 Estimating Glomerular Filtration Rate [DOI]
  5. 5 Enzymatic assays for creatinine: time for action [DOI]
  6. 6 Clinical evaluation of analytical variations in serum creatinine measurements: why laboratories should abandon Jaffe techniques [DOI]
  7. 7 A review on creatinine measurement techniques [DOI]
  8. 8 Recommendations for Improving Serum Creatinine Measurement: A Report from the Laboratory Working Group of the National Kidney Disease Education Program [DOI]
  9. 9 La créatinine: d'hier à aujourd'hui [DOI]
  10. 10 Factors Influencing the Assay of Creatinine [DOI]
  11. 11 Purification and Some Properties of Sarcosine Oxidase from Corynebacterium sp. U-96 [DOI]
  12. 12 Creatinine Assays: Time for Action? [DOI]
  13. 13 Candidate reference method for determining serum creatinine by isocratic HPLC: validation with isotope dilution gas chromatography-mass spectrometry and application for accuracy assessment of routine test kits [DOI]
  14. 14 Mass fragmentography of creatinine proposed as a reference method. [DOI]
  15. 15 [Consequences for clinical biochemists of the modifications of the creatinine-based evaluation of glomerular filtration rate between 2005 and 2008]. [DOI]

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